{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD1084"],"repository":["bioimages"],"figure_sub":["Specimen","Study Component","organisation","Biosample","Associations","Image acquisition"],"pubmed_authors":["Lotte Bang Pedersen"],"additional_accession":[]},"is_claimable":false,"name":"DLG1 functions upstream of SDCCAG3 and IFT20 to control ciliary targeting of polycystin-2 ","description":"Polarized vesicular trafficking directs specific receptors and ion channels to cilia, but the underlying mechanisms are poorly understood. Here we describe a role for DLG1, a core component of the Scribble polarity complex, in regulating ciliary protein trafficking in kidney epithelial cells. Conditional knockout of Dlg1 in mouse kidney caused ciliary elongation and cystogenesis, and cell-based proximity labelling proteomics and fluorescence microscopy showed alterations in the ciliary proteome upon loss of DLG1. Specifically, the retromer-associated protein SDCCAG3, IFT20 and polycystin-2 (PC2) were reduced in cilia of DLG1 deficient cells compared to control cells. This phenotype was recapitulated in vivo and rescuable by re-expression of wildtype DLG1, but not a Congenital Anomalies of ","dates":{"release":"2024-03-15T00:00:00Z","modification":"2024-04-24T09:43:52.333Z","creation":"2024-03-15T09:26:13.97Z"},"accession":"S-BIAD1084","cross_references":{}}