bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1084bioimagesSpecimenStudy ComponentorganisationBiosampleAssociationsImage acquisitionLotte Bang PedersenfalseDLG1 functions upstream of SDCCAG3 and IFT20 to control ciliary targeting of polycystin-2 Polarized vesicular trafficking directs specific receptors and ion channels to cilia, but the underlying mechanisms are poorly understood. Here we describe a role for DLG1, a core component of the Scribble polarity complex, in regulating ciliary protein trafficking in kidney epithelial cells. Conditional knockout of Dlg1 in mouse kidney caused ciliary elongation and cystogenesis, and cell-based proximity labelling proteomics and fluorescence microscopy showed alterations in the ciliary proteome upon loss of DLG1. Specifically, the retromer-associated protein SDCCAG3, IFT20 and polycystin-2 (PC2) were reduced in cilia of DLG1 deficient cells compared to control cells. This phenotype was recapitulated in vivo and rescuable by re-expression of wildtype DLG1, but not a Congenital Anomalies of 2024-03-15T00:00:00Z2024-04-24T09:43:52.333Z2024-03-15T09:26:13.97ZS-BIAD1084