{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD1095"],"repository":["bioimages"],"figure_sub":["Specimen","Study Component","organisation","Biosample","Associations","Image acquisition"],"pubmed_authors":["Xuefei Cheung","Zhongyu Shi"],"additional_accession":[]},"is_claimable":false,"name":"Super-resolution imaging of mice hematopoietic stem cells","description":"Although mitochondrial morphology changes have been consistently associated with hematopoietic stem cells (HSCs) ageing, the specific molecular and cellular mechanisms at play have remained somewhat obscure. Using a live-cell super-resolution (SR) microscopy technique, we have been able to identify different HSCs subsets that feature mitochondria of distinct morphologies and spatial distributions. Integrating SR microscopy with single-cell RNA sequencing enabled us to classify approximately two hundred HSCs from both young and aged mice into five discrete clusters. These clusters show molecular profiles that mirror the varying states of mitochondria. Our integrated approach of RNA biomarker analysis and potential regulon assessment has unveiled hitherto unrecognized functions of GDF15 in m","dates":{"release":"2024-04-04T00:00:00Z","modification":"2024-04-02T10:09:27.383Z","creation":"2024-04-02T10:09:27.383Z"},"accession":"S-BIAD1095","cross_references":{}}