bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1181bioimagesSpecimenImage analysisStudy ComponentorganisationBiosampleAssociationsImage acquisitionCristina Gallego PáramoDina Schneidman-DuhovnyMaria de la Torre-BarrancoRegina FeederleJuan ZouMaria Alba AbadEmilija RuksenaiteOwen R. DaviesA. Arockia JeyaprakashUhn-Soo ChoBethan Medina-PritchardPatrick HeunKazuma ShimanakaJuri RappsilberSang Ho ParkReshma Reshma ThamkachyCarla G. ChiodifalseStructural Basis for Mis18 Complex Assembly: Implications for Centromere Maintenance The centromere, defined by the enrichment of CENP-A (a Histone H3 variant) containing nucleosomes, is a specialised chromosomal locus that acts as a microtubule attachment site. To preserve centromere identity, CENP-A levels must be maintained through active CENP-A loading during the cell cycle. A central player mediating this process is the Mis18 complex (Mis18α, Mis18β and Mis18BP1), which recruits the CENP-A specific chaperone HJURP to centromeres for CENP-A deposition. Here, using a multi-pronged approach, we characterise the structure of the Mis18 complex and show that multiple hetero- and homo-oligomeric interfaces facilitate the hetero-octameric Mis18 complex assembly composed of 4 Mis18α, 2 Mis18β and 2 Mis18BP1. Evaluation of structure-guided/separation-of-function mutants reveals2024-05-20T00:00:00Z2024-05-20T11:38:25.015Z2024-05-20T11:38:25.015ZS-BIAD1181