bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1287bioimagesSpecimenFundingStudy ComponentorganisationBiosampleAssociationsImage acquisitionSudip KhadkaIvan E. IvanovRasmi Cheloor KovilakamChad LiuMadhura BhaveZiwen LiuCarolina AriasManuel D. LeonettiSoorya PradeepBo HuangTalon ChandlerShalin B. MehtaEduardo Hirata MiyasakifalseMantis: high-throughput 4D imaging and analysis of the molecular and physical architecture of cellsUnderstanding the dynamics and interactions of cellular components is crucial for biological research and drug discovery. Current dynamic fluorescence microscopy methods can only image a few fluorescent labels, providing a limited view of these complex processes. We developed Mantis, a high-throughput 3D microscope that maps interactions among components of dynamic cell systems. Mantis combines light-sheet fluorescence imaging of multiple fluorophores with quantitative label-free microscopy and is complemented by shrimPy, our open-source software for high-throughput data acquisition and high-performance analysis. Mantis enabled simultaneous 3D time-lapse imaging of 20 cell lines and quantitative analysis of responses to perturbations like viral infection at single-cell resolution. This app2024-07-30T00:00:00Z2024-08-09T16:45:19.3Z2024-07-23T22:37:32.227ZS-BIAD1287