{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD1292"],"repository":["bioimages"],"figure_sub":["Specimen","Funding","Experiment","Study Component","organisation","Biosample","Associations","Experiments","Image acquisition"],"pubmed_authors":["Martin Offterdinger","Rainer Kurmayer","Rubén Morón Asensio","Anneliese Wiedlroither","David Schuler"],"additional_accession":[]},"is_claimable":false,"name":"Differential Labelling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and Microcystis","description":"The cyanoHAB forming cyanobacteria Microcystis and Planktothrix frequently produce high intracellular amounts of microcystins (MCs) or anabaenopeptins (APs). In this study, chemically modified MCs and APs have been localized on a subcellular level in Microcystis and Planktothrix applying copper-catalyzed alkyne-azide cycloaddition (CuACC). For this purpose, three different non-natural amino acids carrying alkyne or azide moieties were fed to individual P. agardhii strains No371/1 and CYA126/8 as well as to M. aeruginosa strain Hofbauer showing promiscuous incorporation of various amino acid substrates during non-ribosomal peptide synthesis (NRPS). Moreover, CYA126/8 peptide knock-out mutants and non-toxic strain Synechocystis PCC6803 were processed under identical conditions. Simultaneous ","dates":{"release":"2024-09-29T00:00:00Z","modification":"2024-08-16T15:29:14.991Z","creation":"2024-07-25T15:45:13.144Z"},"accession":"S-BIAD1292","cross_references":{}}