bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1292bioimagesSpecimenFundingExperimentStudy ComponentorganisationBiosampleAssociationsExperimentsImage acquisitionMartin OffterdingerRainer KurmayerRubén Morón AsensioAnneliese WiedlroitherDavid SchulerfalseDifferential Labelling of Chemically Modified Peptides and Lipids among Cyanobacteria Planktothrix and MicrocystisThe cyanoHAB forming cyanobacteria Microcystis and Planktothrix frequently produce high intracellular amounts of microcystins (MCs) or anabaenopeptins (APs). In this study, chemically modified MCs and APs have been localized on a subcellular level in Microcystis and Planktothrix applying copper-catalyzed alkyne-azide cycloaddition (CuACC). For this purpose, three different non-natural amino acids carrying alkyne or azide moieties were fed to individual P. agardhii strains No371/1 and CYA126/8 as well as to M. aeruginosa strain Hofbauer showing promiscuous incorporation of various amino acid substrates during non-ribosomal peptide synthesis (NRPS). Moreover, CYA126/8 peptide knock-out mutants and non-toxic strain Synechocystis PCC6803 were processed under identical conditions. Simultaneous 2024-09-29T00:00:00Z2024-08-16T15:29:14.991Z2024-07-25T15:45:13.144ZS-BIAD1292