bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1325bioimagesSpecimenFundingStudy ComponentorganisationBiosampleAssociationsImage acquisitionRainer KurmayerRubén Morón AsensiofalseReal-time observation of clickable cyanopeptide synthesis in bloom-forming cyanobacteria Microcystis and PlanktothrixIn this study time-lapse experiments have been performed using pulsed feeding of non-natural amino acids (non-AAs) in order to observe the build up or decline of azide- or alkyne-modified (clickable) microcystins (MCs) or anabaenopetins (APs) in M. aeruginosa and P. agardhii. Clickable MCs or APs have been subsequently labeled via Copper-catalyzed Azide-Alkyne Cycloaddition (CuAAC) with ALEXA Fluor 488 (A488) and visualized using confocal microscopy analysis.2024-11-29T00:00:00Z2026-06-03T12:13:43.842Z2024-08-16T15:25:32.026ZS-BIAD1325