bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1326bioimagesSpecimenFundingStudy ComponentorganisationBiosampleAssociationsImage acquisitionRainer KurmayerRubén Morón AsensiofalseSubcellular visualization and quantification of cyanotoxins microcystin or anabaenopeptin synthesis in bloom-forming cyanobacteria shows a distinct compartmentationIn this study time-lapse experiments have been performed using pulsed feeding of non-natural amino acids (non-AAs) in order to observe the build up or decline of azide- or alkyne-modified (clickable) microcystins (MCs) or anabaenopetins (APs) in M. aeruginosa and P. agardhii. Clickable MCs or APs have been subsequently labeled via Copper-catalyzed Azide-Alkyne Cycloaddition (CuAAC) with ALEXA Fluor 488 (A488) and visualized using confocal microscopy and advanced image analysis using Imaris 10.1 surfaces function2024-11-29T00:00:00Z2026-01-06T09:22:46.403Z2024-08-16T16:18:43.745ZS-BIAD1326