bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1417bioimagesSpecimenStudy ComponentorganisationBiosampleAssociationsImage acquisitionValeria MarottaAmanda ThomazJudy CoulsonFrancesca QuerquesMark MorganAndrew FieldingAmy PonsfordIan PriorDorota Sabat-PośpiechfalseOTUD6B regulates KIFC1-dependent centrosome clustering and breast cancer cell survival.Cancer cells often display centrosome amplification, requiring the kinesin KIFC1/HSET for centrosome clustering to prevent multipolar spindles and cell death. In parallel siRNA screens of deubiquitinase enzymes, we identify OTUD6B as a positive regulator of KIFC1 expression that is required for centrosome clustering in triple-negative breast cancer (TNBC) cells. OTUD6B can localise to centrosomes and the mitotic spindle and interacts with KIFC1. In OTUD6B-deficient cells, we see increased KIFC1 poly-ubiquitination and premature KIFC1 degradation during mitosis. Depletion of OTUD6B increases multipolar spindles without inducing centrosome amplification. Phenotypic rescue is dependent on OTUD6B catalytic activity and evident upon KIFC1 overexpression. OTUD6B is commonly overexpressed i2024-10-14T00:00:00Z2024-12-15T21:25:47.537Z2024-10-14T05:29:40.574ZS-BIAD1417