bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1483bioimagesSpecimenFundingStudy ComponentorganisationBiosampleAssociationsImage acquisitionalain burettefalseRegional expression of myelin basic protein across postnatal brain development of C57BL/6J miceHealthy brain development hinges on proper myelination. Disruptions in this process result in a variety of neurological disorders. Myelin basic protein (MBP) immunohistochemistry is a key method for studying myelination and myelin-related disorders. Despite MBP studies dating back to the 1980s, there has been a surprising lack of detailed accounts of normal MBP progression across the developing mouse brain. This study presents a detailed spatiotemporal view of MBP distribution across 12 developmental ages from postnatal day 2 to 60. We used an optimized immunohistochemistry protocol to overcome the challenges of myelin's unique lipid-rich composition, enabling more consistent staining across diverse brain structures and developmental stages. 2024-11-26T00:00:00Z2025-02-07T11:12:15.865Z2024-11-19T14:56:11.697ZS-BIAD1483