{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD1665"],"repository":["bioimages"],"figure_sub":["Specimen","Image analysis","Study Component","organisation","Biosample","Associations","Image acquisition"],"pubmed_authors":["Maria Barna","Kathrin Leppek","Zijian Zhang"],"additional_accession":[]},"is_claimable":false,"name":"A versatile toolbox to determine IRES activity in cells and embryonic tissues","description":"Widespread control of gene expression through translation has emerged as a key level of spatiotemporal protein expression regulation. Internal ribosomal entry sites (IRESes) provide a prominent mechanism by which ribosomes can confer greater gene regulation. However, their rigorous functional characterization remains difficult. Here we present a set of technologies in embryos and cells including IRES-mediated translation of circular RNA (circRNA) reporters, single-molecule messenger (m)RNA isoform imaging, PacBio long-read sequencing, and isoform-sensitive mRNA quantification along polysome profiles as a new guideline to understand IRES regulation. We investigate a broad range of cellular IRES RNA elements. We show IRES-dependent translation in circRNAs and the relative expression, localiz","dates":{"release":"2025-02-26T00:00:00Z","modification":"2025-02-26T16:25:01.507Z","creation":"2025-02-26T16:25:01.507Z"},"accession":"S-BIAD1665","cross_references":{}}