bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1665bioimagesSpecimenImage analysisStudy ComponentorganisationBiosampleAssociationsImage acquisitionMaria BarnaKathrin LeppekZijian ZhangfalseA versatile toolbox to determine IRES activity in cells and embryonic tissuesWidespread control of gene expression through translation has emerged as a key level of spatiotemporal protein expression regulation. Internal ribosomal entry sites (IRESes) provide a prominent mechanism by which ribosomes can confer greater gene regulation. However, their rigorous functional characterization remains difficult. Here we present a set of technologies in embryos and cells including IRES-mediated translation of circular RNA (circRNA) reporters, single-molecule messenger (m)RNA isoform imaging, PacBio long-read sequencing, and isoform-sensitive mRNA quantification along polysome profiles as a new guideline to understand IRES regulation. We investigate a broad range of cellular IRES RNA elements. We show IRES-dependent translation in circRNAs and the relative expression, localiz2025-02-26T00:00:00Z2025-02-26T16:25:01.507Z2025-02-26T16:25:01.507ZS-BIAD1665