bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD1695bioimagesSpecimenStudy ComponentorganisationBiosampleAssociationsImage acquisitionPetra RiegerováKarina Pombo-GarciaJuan M. Iglesias-ArtolaKristin BöhligMilena SchuhmacherHjoerdis Mathilda LennartzVannuruswamy GarikapatiAlf HonigmannRadek ŠachlBjörn DrobotMartin HofAndrej ShevchenkoAnnett LohmannAndré NadlerCristina Jiménez LópezPavel BarahtjanKai SchuhmannKatelyn C. CookfalseQuantitative imaging of lipid transport in mammalian cells - endocytosis inhibitorsTo test the predominant role of the non-vesicular route for retrograde transport in U-2 OS cells, we inhibited vesicular trafficking using Brefeldin A, which blocks COPI coat formation at Golgi membranes and Wortmannin, which broadly affects endosome formation and trafficking and quantified transport of SM(Y), PE(18:1/Y), PC(Y:16:0), PC(Y/20:4) using the lipid imaging pipeline. Paper: Iglesias-Artola et al. Quantitative imaging of lipid transport in mammalian cells. Nature (2025). https://doi.org/10.1038/s41586-025-09432-x2025-03-12T00:00:00Z2025-08-22T12:16:46.241Z2025-03-12T14:57:24.232ZS-BIAD1695