{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Emily D. Cosco"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD27"],"repository":["bioimages"],"additional_accession":["https://gitlab.com/brunslab/ccda"],"figure_sub":["Experiment 1","Experiment 2","Organization","Experiment 10","Experiment 11","Experiment 9","Experiment 7","Experiment 8","Experiment 5","Experiment 6","Experiment 3","Experiment 4"],"pubmed_authors":["Emily D. Cosco","Ellen M. Sletten","Oliver T. Bruns"]},"is_claimable":false,"name":"Shortwave infrared polymethine fluorophores matched to excitation lasers enable non-invasive, multicolour in vivo imaging in real time","description":"High-resolution, multiplexed experiments are a staple in cellular imaging. Analogous experiments in animals are challenging, however, due to substantial  scattering and autofluorescence in tissue at visible (350–700?nm) and near-infrared (700–1,000?nm) wavelengths. Here, we enable real-time, non-invasive multicolour imaging experiments in animals through the design of optical contrast agents for the shortwave infrared (SWIR, 1,000–2,000?nm) region and complementary advances in imaging technologies. We developed tunable, SWIR-emissive flavylium polymethine dyes and established relationships between structure and photophysical properties for this class of bright SWIR contrast agents. In parallel, we designed an imaging system with variable near-infrared/SWIR excitation and single-channel det","dates":{"release":"2020-10-03T00:00:00Z","modification":"2023-04-07T19:21:56.564Z","creation":"2020-09-08T11:02:12Z"},"accession":"S-BIAD27","cross_references":{}}