bioimagesUnknownNika Zaveršekhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD2741bioimagesSpecimenImage analysisFundingStudy ComponentBiosampleorganisationAssociationsImage acquisitionNika ZaveršekJerica SabotičMaja CafSlavko KraljfalseAnisotropic Magnetic Particles with Different Dimensions, Morphologies and Surface Grafting for Magnetic Field-Assisted Biofilm RemovalBacteria in biofilms are shielded from environmental stressors and therefore show strong resistance to conventional removal strategies, including chemical disinfectants and antibiotics. In this work, we systematically evaluated emerging biofilm removal approaches on Listeria innocua biofilms using nanomaterial-based methods. Anisotropic magnetic particles (AMPs), made from iron oxide, and antibacterial silver nanoparticles were used to assess the potential of nanostructure-mediated disruption. We investigated how AMP properties—specifically surface roughness and particle size—affect biofilm removal under magnetic actuation, using both classical colony-forming unit quantification and fluorescence-based measurements. Although individual AMP characteristics contributed only modestly to biofilm disruption, a synergistic effect was observed when AMPs were grafted with silver nanoparticles, as also shown by scanning electron microscopy. Additionally, comparative assays on other resilient biofilm-forming species, including Enterococcus faecalis and Candida albicans, further confirmed the pronounced strain-dependent variability of silver-based treatments, emphasizing the need for hybrid magneto–chemical strategies to achieve consistent and robust biofilm control. 2026-05-20T00:00:00Z2026-05-20T01:01:37.217Z2026-01-06T12:19:49.808ZS-BIAD2741