{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"species":["Mus musculus (mouse)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD338"],"repository":["bioimages"],"figure_sub":["Study Component","organisation","Study Protocols"],"pubmed_authors":["Irene Robles Rebollo","Matthias Merkenschlager"],"additional_accession":[]},"is_claimable":false,"name":"smFISH of genes and enhancers during the LPS response of wild-type and cohesin-deficient macrophages","description":"Transcription is an intermittent process that occurs in bursts. The frequency and the number of messenger RNA (mRNA) produced by these bursts determine expression levels and shape cell-to-cell variability. Productive encounters between enhancers and promoters control burst dynamics, which the cohesin complex can facilitate through loop extrusion. Cohesin is a ring-shaped complex involved in enhancer-promoter interactions. Without cohesin, bone-marrow-derived macrophages' transcriptional response to LPS is severely impaired. Here, we used bDNA smFISH to detect mature and nascent inducible gene mRNA, as well as enhancer transcripts.","dates":{"release":"2022-03-31T00:00:00Z","modification":"2023-03-10T18:42:39.07Z","creation":"2022-02-22T15:03:45.955Z"},"accession":"S-BIAD338","cross_references":{}}