bioimagesUnknownMus musculus (mouse)https://www.ebi.ac.uk/biostudies/studies/S-BIAD338bioimagesStudy ComponentorganisationStudy ProtocolsIrene Robles RebolloMatthias MerkenschlagerfalsesmFISH of genes and enhancers during the LPS response of wild-type and cohesin-deficient macrophagesTranscription is an intermittent process that occurs in bursts. The frequency and the number of messenger RNA (mRNA) produced by these bursts determine expression levels and shape cell-to-cell variability. Productive encounters between enhancers and promoters control burst dynamics, which the cohesin complex can facilitate through loop extrusion. Cohesin is a ring-shaped complex involved in enhancer-promoter interactions. Without cohesin, bone-marrow-derived macrophages' transcriptional response to LPS is severely impaired. Here, we used bDNA smFISH to detect mature and nascent inducible gene mRNA, as well as enhancer transcripts.2022-03-31T00:00:00Z2023-03-10T18:42:39.07Z2022-02-22T15:03:45.955ZS-BIAD338