bioimagesUnknownBenjamin F. Combeshttps://www.ebi.ac.uk/biostudies/studies/S-BIAD3402bioimagesSpecimenImage analysisStudy ComponentBiosampleorganisationImage correlationAssociationsImage acquisition Liqin YangYi ChenRoger M. NitschMarco ReisertTian ZengAlicia K. RosenauMartin T. HenrichWolfgang H. OertelSepp KollmorgenRuiqing NiRong ChenFanni F. GeiblJie LuXunbin WeiHongjiang WeiTheofanis KarayannisMaria KaratsoliTed M. DawsonChristoph HockBenjamin F. CombesSihan DongValina L. DawsonPeter R NilssonDaniel RazanskyAxel RomingerKuangyu Shifalse[Historical Versions Pending Deletion] Multiscale brain-wide mapping of α-synuclein-driven dopaminergic degeneration and white matter impairment in α-synucleinopathy miceCitation: [Multi-modal imaging analysis of Parkinsonian mice reveals region-specific alterations in the substantia nigra] The spatiotemporal relationships among α-synuclein inclusion formation, dopaminergic degeneration, and white matter microstructural changes remain poorly defined. To address this, we unilaterally injected preformed fibrils (PFFs) or monomeric α-synuclein into the substantia nigra pars compacta (SNc) of wild-type mice. At 12 and 20 weeks post-injection (wpi), we performed ex vivo magnetic resonance imaging (MRI) at 9.4T to assess microstructural, volumetric and paramagnetic changes, along with light-sheet microscopy (LSM) to map α- synuclein aggregates, and dopaminergic neuron at single-cell resolution across the whole brain. We developed a Python-based, automated regis2026-05-09T00:00:00Z2026-08-07T01:04:04.828Z2026-05-09T09:48:57.734ZS-BIAD3402