bioimagesUnknownSiddharth Acharyahttps://www.ebi.ac.uk/biostudies/studies/S-BIAD3409bioimagesSpecimenImage analysisFundingStudy ComponentBiosampleorganisationImage correlationAssociationsImage acquisitionJoshua J. FisherSahan JayatissaRoger SmithLucy A. BarthoTia M. SmithJoanna L. JamesZlatan TrifunovicTu'uhevaha J. Kaitu'u-LinoJames C. BouwerSiddharth AcharyaVeronica B. BothaJohn E. SchjenkenAnthony V. PerkinsEric HanssenKirsty G. PringlefalseElectron tomography reveals mitochondrial network and cristae remodelling during cell differentiation in the human placenta (cryo-electron tomography)Mitochondria adapt their structure through fusion and fission, yet how their morphology and cristae architecture change as cells differentiate remains unclear. The human placenta is a valuable model for studying mitochondrial diversity within a single tissue. As epithelial trophoblast cells of the placenta differentiate, their accompanying mitochondria morphologically and functionally transform. We used array tomography to characterise mitochondrial volume and network complexity. Cryo-electron tomography revealed two distinct subpopulations of mitochondria within progenitor cytotrophoblasts, and a singular, homogeneous population in the differentiated syncytiotrophoblast. We showcased the 3D topology of individual cristae through a standardised metric of “curvedness”. Proteomic analysis of2026-05-15T00:00:00Z2026-08-13T13:49:20.874Z2026-05-11T03:43:04.015ZS-BIAD3409