{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"species":["Homo sapiens (human)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD368"],"repository":["bioimages"],"figure_sub":["Protocols","Funding","Study Component","organisation"],"pubmed_authors":["Ying Li","Bastiaan Dekker","Sergey V. Venev","Denis L. Lafontaine","A. Nicole Fox","Erica M Hildebrand","Yu Liu","Job Dekker","Kirill Polovnikov","Leonid Mirny"],"additional_accession":[]},"is_claimable":false,"name":"Directed Topoisomerase II activity disentangles interphase compartment domains","description":"The topological state of chromosomes determines their mechanical properties, dynamics, and function. Recent work indicated that interphase chromosomes are largely free of entanglements. Here, we use Hi-C, polymer simulations and multi-contact 3C, and propose that, in contrast, mitotic chromosomes are self-entangled. We explore how a mitotic self-entangled state is converted into an unentangled interphase state during mitotic exit. Most mitotic entanglements are removed during anaphase/telophase, with remaining ones removed during early G1, in a Topoisomerase II-dependent process. Polymer models suggest a two-stage disentanglement pathway: first, decondensation of mitotic chromosomes with remaining condensin loops produces entropic forces that bias Topoisomerase II activity towards decatena","dates":{"release":"2023-03-10T00:00:00Z","modification":"2024-03-07T20:16:12.23Z","creation":"2022-03-11T15:46:07.77Z"},"accession":"S-BIAD368","cross_references":{}}