bioimagesUnknownDavid Constanthttps://www.ebi.ac.uk/biostudies/studies/S-BIAD3732bioimagesSpecimenImage analysisFundingStudy ComponentBiosampleorganisationAssociationsImage acquisitionTimothy NiceDavid ConstantfalseIntestinal plasmacytoid dendritic cells preferentially produce interferon lambda contributing to localized innate immune responsesThe healthy intestine maintains homeostasis in part via immune responses to microbiota, which includes basal production of interferon cytokines. Previous work showed that Type III Interferon (IFN-λ) stimulates localized pockets of interferon-stimulated genes (ISGs) in the adult mouse intestinal epithelium at homeostasis that provide preemptive protection from viral pathogens. Here, we demonstrate that a major source of homeostatic IFN-λ production in the intestine is a population of epithelium-associated plasmacytoid dendritic cells (pDC). Expansion of the pDC population increases epithelial ISG expression at homeostasis, suggesting the abundance of these cells is a limiting factor in IFN-λ responses. On the other hand, depletion of pDC or bone marrow reconstitution with IFN-λ-deficient pD2026-08-14T00:00:00Z2026-08-14T01:00:49.321Z2026-08-03T17:26:25.328ZS-BIAD3732