bioimagesUnknownPhyscomitrella patens (moss)https://www.ebi.ac.uk/biostudies/studies/S-BIAD466bioimagesProtocolsStudy ComponentorganisationStudy ProtocolsWaltraud X. SchulzeNeda S. Kazemein JasemiMoritz SchladtPeng XuManuel MirasZhaoxia ZhangWolfgang BaumeisterWolf B. FrommerSebastian HänschSven GombosLin XiJona EjikeMathieu PottierRüdiger SimonMarcel DickmannsUlla NeumannVicky HoweFranziska KuttigfalseA high confidence Physcomitrium patens plasmodesmata proteome by iterative scoring and validation reveals diversification of cell wall proteins during evolution Cells of multicellular organisms exchange nutrients, building blocks and information. In animals, this happens via gap junctions, in plants via plasmodesmata (PD). PD have striking properties, translocating a large range of molecules from ions, to metabolites, RNA and proteins up to 40 kDa. PD are hard to characterize due to being deeply embedded into cell walls and the presence of several membranes. While previous studies of protein composition of PD from angiosperms identified large lists of proteins, few were validated. Here, we developed a PD scoring approach in conjunction with systematic localization on a large scale to define a high-confidence PD proteome of Physcomitrium patens. This high confidence PD proteome comprises nearly 300 proteins, which together with the bona fide PD pro2023-01-31T00:00:00Z2023-01-16T15:44:59.836Z2022-05-27T12:56:27.838ZS-BIAD466