bioimagesUnknownStreptococcus pneumoniaehttps://www.ebi.ac.uk/biostudies/studies/S-BIAD477bioimagesProtocolsStudy ComponentorganisationStudy ProtocolsLiselot DewachterJan-Willem VeeningfalseImages for "Amoxicillin-resistant Streptococcus pneumoniae can be resensitized by targeting the mevalonate pathway as indicated by sCRilecs-seq"Antibiotic resistance in the important opportunistic human pathogen Streptococcus pneumoniae is on the rise. This is particularly problematic in the case of the β-lactam antibiotic amoxicillin, which is the first-line therapy. It is therefore crucial to uncover targets that would kill or resensitize amoxicillin-resistant pneumococci. To do so, we developed a genome-wide, single-cell based, gene silencing screen using CRISPR interference called sCRilecs-seq (subsets of CRISPR interference libraries extracted by fluorescence activated cell sorting coupled to next generation sequencing). Since amoxicillin affects growth and division, sCRilecs-seq was used to identify targets that are responsible for maintaining proper cell size. Our screen revealed that downregulation of the mevalonate pathwa2022-06-09T00:00:00Z2023-04-08T16:17:10.604Z2022-06-10T12:38:46.794ZS-BIAD477