bioimagesUnknownHomo sapiens (human)https://www.ebi.ac.uk/biostudies/studies/S-BIAD565bioimagesProtocolsStudy ComponentorganisationStudy ProtocolsGarry P. NolanWolfgang HuberAlexander BrobeilChristian M. SchürchLaura Llao-CidNora LiebersSascha DietrichDonnacha FitzgeraldBerit J. BrinkmannVerena PasseriniCarsten Müller-TidowMartina SeiffertOliver WeigertMarc A. BärtschMareike KnollHarald VöhringerTobias RoiderFelix CzernilofskyPeter-Martin Bruchfalse[Reserach Data] Multimodal and spatially resolved profiling of lymph node-derived T-cells in nodal B-cell lymphomaT-cell-engaging immunotherapies have improved the treatment of nodal B-cell lymphoma, but responses vary highly. Future improvements of such therapies require better understanding of the variety of lymphoma-infiltrating T-cells. We employed single-cell RNA and T-cell receptor sequencing alongside quantification of surface proteins, flow cytometry and multiplexed immunofluorescence on 101 lymph nodes from healthy controls, and patients with diffuse large B-cell, mantle cell, follicular, or marginal zone lymphoma. Our multimodal and spatially resolved dataset revealed entity-specific alterations of the T-cell microenvironment. 2023-08-31T00:00:00Z2023-03-03T12:15:36.921Z2022-10-09T15:33:38.131ZS-BIAD565