bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD609bioimagesSpecimenImage analysisStudy ComponentorganisationBiosampleImage correlationAssociationsImage acquisitionRoosmarijn E. VandenbrouckeArnout BruggemanClint De NolfCharysse VandendriesscheElien Van WonterghemJunhua XieLars VereeckeGriet Van ImschootfalseThe importance of the blood-cerebrospinal fluid interface in the gut-brain axis and Aβ pathologyIn this study, we found that mice lacking gut microbiota displayed increased blood-cerebrospinal fluid (CSF) barrier permeability associated with disorganized tight junctions (TJs), which can be rescued by recolonization with gut microbiota or supplementation with short-chain fatty acids (SCFAs). We further confirmed that the vagus nerve plays an important role in this process but SCFAs can also bypass the vagus nerve and affect blood-CSF barrier functionality. Strikingly, the administration of SCFAs in AppNL-G-F AD mice also improved the localization of TJs in the blood-CSF barrier. This was accompanied by a pronounced reduction in β-amyloid (Aβ) burden, which could be achieved by regulating microglial phenotype. Altogether, our results suggest that modulating the microbiota or administer2022-12-14T00:00:00Z2023-01-14T20:09:46.908Z2023-01-14T20:09:46.908ZS-BIAD609