bioimagesUnknownHomo sapiens (human)https://www.ebi.ac.uk/biostudies/studies/S-BIAD621bioimagesProtocolsStudy ComponentorganisationStudy ProtocolsOliver Facklerfalsesurce data fig 3b collagen from Gallucci et Immature Dendritic Cells (iDCs) migrate in microenvironments with distinct cell and extracellular matrix densities in vivo and contribute to HIV-1 dissemination and mounting of antiviral immune responses. Here, we find that, compared to standard 2D suspension cultures, 3D collagen as tissue-like environment alters iDC properties and their response to HIV-1 infection. iDCs adopt an elongated morphology with increased deformability in 3D collagen at unaltered activation, differentiation, cytokine secretion or responsiveness to LPS. While 3D collagen reduces HIV-1 particle uptake by iDCs, fusion efficiency is increased to elevate productive infection rates due to elevated cell surface exposure of the HIV-1 binding receptor DC-SIGN. In contrast, 3D collagen reduces HIV transfer to CD4 T-cells 2023-02-08T00:00:00Z2023-02-08T20:28:43.196Z2023-02-08T20:28:43.196ZS-BIAD621