bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD814bioimagesSpecimenStudy ComponentorganisationBiosampleAssociationsImage acquisitionVerena M. PradeJun WangAxel WalchAnnette FeuchtingerThomas KunzkeSabina BerezowskaJian ShenPhilipp ZensNa SunfalseSpatial metabolomics identifies distinct tumor-specific and stroma-specific subtypes in patients with lung squamous cell carcinomaMolecular subtyping of lung squamous cell carcinoma (LUSC) has been performed at the genomic, transcriptomic, and proteomic level. However, LUSC stratification based on tissue metabolomics is still lacking. Combining high-mass-resolution imaging mass spectrometry with consensus clustering, four tumor- and four stroma-specific subtypes with distinct metabolite patterns were identified in 330 LUSC patients. The first tumor subtype T1 negatively correlated with DNA damage and immunological features including CD3, CD8, and PD-L1. The same features positively correlated with the tumor subtype T2. Tumor subtype T4 was associated with high PD-L1 expression. Compared with the status of subtypes T1 and T4, patients with subtype T3 had improved prognosis, and T3 was an independent prognostic factor 2023-07-11T00:00:00Z2023-07-12T19:15:27.336Z2023-07-11T01:10:03.791ZS-BIAD814