{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD848"],"repository":["bioimages"],"additional_accession":["https://doi.org/10.17867/10000105"],"figure_sub":["Specimen","Image analysis","Study Component","organisation","Biosample","Associations","Image acquisition"],"pubmed_authors":["Image Data Resource (IDR)"]},"is_claimable":false,"name":"Systematic morphological profiling of human gene and allele function via Cell Painting (OME-NGFF)","description":"OME-NGFF converted study from idr0033.  We hypothesized that human genes and disease-associated alleles might be systematically functionally annotated using morphological profiling of cDNA constructs, via a microscopy-based Cell Painting assay. Indeed, 50% of the 220 tested genes yielded detectable morphological profiles, which grouped into biologically meaningful gene clusters consistent with known functional annotation (e.g., the RAS-RAF-MEK-ERK cascade). We used novel subpopulation-based visualization methods to interpret the morphological changes for specific clusters. This unbiased morphologic map of gene function revealed TRAF2/c-REL negative regulation of YAP1/WWTR1-responsive pathways. We confirmed this discovery of functional connectivity between the NF-kB pathway and Hippo pathwa","dates":{"release":"2023-08-15T00:00:00Z","modification":"2023-08-21T07:36:04.488Z","creation":"2023-08-15T16:44:47.582Z"},"accession":"S-BIAD848","cross_references":{}}