bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD848bioimageshttps://doi.org/10.17867/10000105SpecimenImage analysisStudy ComponentorganisationBiosampleAssociationsImage acquisitionImage Data Resource (IDR)falseSystematic morphological profiling of human gene and allele function via Cell Painting (OME-NGFF)OME-NGFF converted study from idr0033. We hypothesized that human genes and disease-associated alleles might be systematically functionally annotated using morphological profiling of cDNA constructs, via a microscopy-based Cell Painting assay. Indeed, 50% of the 220 tested genes yielded detectable morphological profiles, which grouped into biologically meaningful gene clusters consistent with known functional annotation (e.g., the RAS-RAF-MEK-ERK cascade). We used novel subpopulation-based visualization methods to interpret the morphological changes for specific clusters. This unbiased morphologic map of gene function revealed TRAF2/c-REL negative regulation of YAP1/WWTR1-responsive pathways. We confirmed this discovery of functional connectivity between the NF-kB pathway and Hippo pathwa2023-08-15T00:00:00Z2023-08-21T07:36:04.488Z2023-08-15T16:44:47.582ZS-BIAD848