{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":[null],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BIAD885"],"repository":["bioimages"],"figure_sub":["Specimen","Image analysis","Study Component","organisation","Biosample","Associations","Image acquisition"],"pubmed_authors":["Image Data Resource (IDR)"],"additional_accession":[]},"is_claimable":false,"name":"High content screen for genes whose downregulation by RNAi affects acccumulation of p53-binding protein 1 (53BP1) at sites of double strand breaks (OME-NGFF)","description":"OME-NGFF converted study from idr0010.  DNA double-strand breaks (DSBs) not only interrupt the genetic information, but also disrupt the chromatin structure, and both impairments require repair mechanisms to ensure genome integrity. We showed previously that RNF8-mediated chromatin ubiquitylation protects genome integrity by promoting the accumulation of repair factors at DSBs. Here, we provide evidence that, while RNF8 is necessary to trigger the DSB-associated ubiquitylations, it is not sufficient to sustain conjugated ubiquitin in this compartment. We identified RNF168 as a novel chromatin-associated ubiquitin ligase with an ability to bind ubiquitin. We show that RNF168 interacts with ubiquitylated H2A, assembles at DSBs in an RNF8-dependent manner, and, by targeting H2A and H2AX, ampl","dates":{"release":"2023-09-06T00:00:00Z","modification":"2024-02-06T14:17:37.12Z","creation":"2023-09-06T15:57:04.634Z"},"accession":"S-BIAD885","cross_references":{}}