bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD885bioimagesSpecimenImage analysisStudy ComponentorganisationBiosampleAssociationsImage acquisitionImage Data Resource (IDR)falseHigh content screen for genes whose downregulation by RNAi affects acccumulation of p53-binding protein 1 (53BP1) at sites of double strand breaks (OME-NGFF)OME-NGFF converted study from idr0010. DNA double-strand breaks (DSBs) not only interrupt the genetic information, but also disrupt the chromatin structure, and both impairments require repair mechanisms to ensure genome integrity. We showed previously that RNF8-mediated chromatin ubiquitylation protects genome integrity by promoting the accumulation of repair factors at DSBs. Here, we provide evidence that, while RNF8 is necessary to trigger the DSB-associated ubiquitylations, it is not sufficient to sustain conjugated ubiquitin in this compartment. We identified RNF168 as a novel chromatin-associated ubiquitin ligase with an ability to bind ubiquitin. We show that RNF168 interacts with ubiquitylated H2A, assembles at DSBs in an RNF8-dependent manner, and, by targeting H2A and H2AX, ampl2023-09-06T00:00:00Z2024-02-06T14:17:37.12Z2023-09-06T15:57:04.634ZS-BIAD885