bioimagesUnknownhttps://www.ebi.ac.uk/biostudies/studies/S-BIAD963bioimagesSpecimenStudy ComponentorganisationBiosampleAssociationsImage acquisitionRuiyao CaiJosé María MateosPhilipp BethgeEsther StoeckliValentino AerneAdriano AguzziSoeren S. LienkampAnna Maria ReussGabriela R. AraujoTaiyo YamamotoShan ZhaoAli ErtürkPatricia SchmidMartina SchaettinNikita VladimirovFabian VoigtThomas NaertSven HildebrandFritjof HelmchenAlard RoebroeckUrs ZieglerDominik GroosLaura BaudisfalseThe Benchtop mesoSPIM: a next-generation open-source light-sheet microscope for large cleared samplesIn 2015, we launched the mesoSPIM initiative (www.mesospim.org), an open-source project for making light-sheet microscopy of large cleared tissues more accessible. Meanwhile, the demand for imaging larger samples at higher speed and resolution has increased, requiring major improvements in the capabilities of light-sheet microscopy. Here, we introduce the next-generation mesoSPIM ("Benchtop") with significantly increased field of view, improved resolution, higher throughput, more affordable cost and simpler assembly compared to the original version. We developed a new method for testing objectives, enabling us to select detection objectives optimal for light-sheet imaging with large-sensor sCMOS cameras. The new mesoSPIM achieves high spatial resolution (1.5 µm laterally, 3.3 µm axially) a2023-12-15T00:00:00Z2023-12-13T13:40:12.359Z2023-12-07T13:36:23.49ZS-BIAD963