{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Christian Gabriel"],"species":["Homo sapiens (human)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BSST630"],"repository":["bioimages"],"figure_sub":["Organization"],"pubmed_authors":["Christian Gabriel"],"additional_accession":[]},"is_claimable":false,"name":"Circadian time series of dexamethasone synchronized U-2 OS knock-in cell expressing fluorescent fusion proteins of CRY1 and/or PER2 ","description":"U-2 OS knock-in cells were genereated using CRISPR to express CRY1 and/or PER2 fused to either mScarlet-I or mClover3 from the endogenous promoter. Cells were synchronized with dexamethasone and imaged for 71h with an sampling interval of 1/h, starting 2 h after synchronization. Files are .nd2 and contain 3 channels: (1)RFP channel, (2)YFP channel, (3) DIC channel. All files are stacks with 71 timepoints. nd2 file image stacks can be opened using the freeware Fiji/ImageJ, but also contain alot of metadata. For each cell line, 10 imaging regions are provided.\nThe cells identifiers are as follows: 42c5: CRY1-mClover3, 44c5: CRY1-mScarlet-I, 56c1: PER2-mClover3. 58b59: PER2-mScarlet-I. 80c16: CRY1-mClover3/PER2-mScarlet-I.\nAdditionally, 4 imagiong areas without cells are provided, which were ","dates":{"release":"2021-04-15T00:00:00Z","modification":"2023-04-09T17:42:42.349Z","creation":"2021-04-15T12:28:14Z"},"accession":"S-BSST630","cross_references":{}}