{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Christian Gabriel"],"species":["Homo sapiens (human)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BSST631"],"repository":["bioimages"],"figure_sub":["Organization"],"pubmed_authors":["Christian Gabriel"],"additional_accession":[]},"is_claimable":false,"name":"24h time series of  U-2 OS knock-in cells expressing fluorescent fusion proteins of CRY1 and/or PER2, left untreated or after shRNA mediated knock-down of either CRY1 or PER2, respectively.","description":"U-2 OS knock-in cells were genereated using CRISPR to express CRY1 and/or PER2 fused to either mScarlet-I or mClover3 from the endogenous promoter. Cells were either left untreated or trasnduced with lentivirus expressing shRNA against either PER2 or CRY1 (pGIPZ clones V2LHS_172866 (CRY1) V2LHS_52938 (PER2) with mutated tGFP).\nCells were synchronized by washing them in cold PBS. Imging was performed on a Nikon widefield microscope for 24 h with an sampling interval of 1/h, starting 2.5 h after synchronization. Files are .nd2 and contain 4 channels:(1)RFP channel, (2)YFP channel, (3) a YFP/RFP channel (not used) (4) DIC channel. All files are stacks with 24 timepoints. nd2 file image stacks can be opened using the freeware Fiji/ImageJ, but also contain alot of metadata. For each cell line a","dates":{"release":"2021-04-18T00:00:00Z","modification":"2023-04-08T18:54:17.898Z","creation":"2021-04-18T19:59:51Z"},"accession":"S-BSST631","cross_references":{}}