{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["154","7"],"submitter":["Mike Heilemann"],"species":["Homo sapiens (human)"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-BSST712"],"repository":["bioimages"],"figure_sub":["Organization"],"pubmed_authors":["Mike Heilemann","Marina S Dietz","Johanna V Rahm","Marie-Lena IE Harwardt"],"additional_accession":[]},"is_claimable":false,"name":"Single-particle tracking (SPT) data of MET with a non-activating Fab ligand and the activating ligand InlB321 recorded in HeLa cells","description":"Universal point accumulation for imaging in nanoscale topography (uPAINT) was applied to measure the dynamics of the MET receptor in living HeLa cells. For the resting receptor, an ATTO 647N-labeled, non-activating Fab antibody fragment was used. The ligand-bound state was probed using the InlB321 ligand site-specifically labeled with ATTO 647N which was fully functional. Imaging was performed in total internal reflection fluorescence (TIRF) mode using an N-STORM microscope (Nikon, Japan).","dates":{"release":"2021-09-23T00:00:00Z","modification":"2023-04-19T13:54:42.663Z","creation":"2021-09-23T17:19:56Z"},"accession":"S-BSST712","cross_references":{}}