{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Rebecca C. Burgess"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200810055"],"attach_to":["JCB"],"legend":["Cells containing Rad52-RFP and YFP-Srs2 and a PCNA mutant that is not able to be sumoylated were imaged through 11 z-sections at 0.3µm intervals. In small-budded (S phase) cells, Srs2 does not form multiple small replication foci.  However, Srs2 retains the ability to colocalize with Rad52 in recombination foci.<br /><br />To view DIC only, turn off Channels 1 and 2.  Turn on Channel 0. For grayscale, uncheck the color\" box (above \"zoom\").\"","Cells expressing Rad52-RFP, YFP-Srs2 and CFP-Pol30 were imaged through 11 z-sections at 0.3µm intervals. A subset of Srs2 foci colocalize with Rad52, particularly in large budded (G2/M) cells.  However, in small-budded (S phase) cells, Srs2 foci colocalize with PCNA replication foci.<br /><br />To view DIC only, turn off Channels 1, 2, and 3.  Turn on Channel 0. For grayscale, uncheck the color\" box (above \"zoom\").\"","Cells containing Rad52-RFP and YFP-Srs2 and a deletion of RAD51 were imaged in 11 z-sections at 0.3µm intervals.  Srs2 is not only capable of localizing to replication foci in the absence of Rad51, but also localizes to recombination foci. <br /><br />To view DIC only, turn off Channels 1, and 2.  Turn on Channel 0. For grayscale, uncheck the color\" box (above \"zoom\").\"","Cells containing Rad52-RFP and YFP-Srs2 were imaged through 11 z-sections at 0.3µm intervals. In small-budded (S phase) cells, Srs2 forms multiple small replication foci.  Srs2 also colocalizes often with Rad52 in recombination foci.<br /><br />To view DIC only, turn off Channels 1 and 2.  Turn on Channel 0. For grayscale, uncheck the color\" box (above \"zoom\").\""],"repository":["bioimages"],"figure_sub":["Image 1573 (Fig 1 - B)","Fig 1 - A","Fig 1 - B","Fig 1 - C","Image 1574 (Fig 1 - B)","Image 1570 (Fig 1 - A)","Image 1575 (Fig 1 - B)","Image 1571 (Fig 1 - A)","Fig 1","Image 1572 (Fig 1 - B)","Image 1576 (Fig 1 - C)"],"pubmed_authors":["Veronika Altmannova","Patrick Sung","Michael Lisby","Rebecca C. Burgess","Lumir Krejci","Rodney Rothstein"],"additional_accession":[]},"is_claimable":false,"name":"Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo","description":null,"dates":{"release":"2009-06-08T11:17:04Z","modification":"2018-11-29T11:17:04Z","creation":"2018-11-29T11:17:04Z"},"accession":"S-JCBD-200810055","cross_references":{"doi":["10.1083/jcb.200810055"]}}