bioimagesUnknownRebecca C. BurgessThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200810055JCB

Cells containing Rad52-RFP and YFP-Srs2 and a PCNA mutant that is not able to be sumoylated were imaged through 11 z-sections at 0.3µm intervals. In small-budded (S phase) cells, Srs2 does not form multiple small replication foci. However, Srs2 retains the ability to colocalize with Rad52 in recombination foci.<br /><br />To view DIC only, turn off Channels 1 and 2. Turn on Channel 0. For grayscale, uncheck the color" box (above "zoom")."

Cells expressing Rad52-RFP, YFP-Srs2 and CFP-Pol30 were imaged through 11 z-sections at 0.3µm intervals. A subset of Srs2 foci colocalize with Rad52, particularly in large budded (G2/M) cells. However, in small-budded (S phase) cells, Srs2 foci colocalize with PCNA replication foci.<br /><br />To view DIC only, turn off Channels 1, 2, and 3. Turn on Channel 0. For grayscale, uncheck the color" box (above "zoom")."

Cells containing Rad52-RFP and YFP-Srs2 and a deletion of RAD51 were imaged in 11 z-sections at 0.3µm intervals. Srs2 is not only capable of localizing to replication foci in the absence of Rad51, but also localizes to recombination foci. <br /><br />To view DIC only, turn off Channels 1, and 2. Turn on Channel 0. For grayscale, uncheck the color" box (above "zoom")."

Cells containing Rad52-RFP and YFP-Srs2 were imaged through 11 z-sections at 0.3µm intervals. In small-budded (S phase) cells, Srs2 forms multiple small replication foci. Srs2 also colocalizes often with Rad52 in recombination foci.<br /><br />To view DIC only, turn off Channels 1 and 2. Turn on Channel 0. For grayscale, uncheck the color" box (above "zoom")."

bioimagesImage 1573 (Fig 1 - B)Fig 1 - AFig 1 - BFig 1 - CImage 1574 (Fig 1 - B)Image 1570 (Fig 1 - A)Image 1575 (Fig 1 - B)Image 1571 (Fig 1 - A)Fig 1Image 1572 (Fig 1 - B)Image 1576 (Fig 1 - C)Veronika AltmannovaPatrick SungMichael LisbyRebecca C. BurgessLumir KrejciRodney RothsteinfalseLocalization of recombination proteins and Srs2 reveals anti-recombinase function in vivo2009-06-08T11:17:04Z2018-11-29T11:17:04Z2018-11-29T11:17:04ZS-JCBD-20081005510.1083/jcb.200810055