bioimagesUnknownIsabelle DupinThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200812034JCBAstrocytes were plated onto fibronectin printed micropatterns in the presence of calcium and were subsequently incubated for 2h in calcium-free medium and then for 5h in calcium-containing medium. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2B). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2C). The typical intracellular organization of the cell in each condition is depicted (Figure 2A). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2A).Grouped cells with no free edge. Primary rat astrocytes were plated onto fibronectin-printed micropatterns and the distances between the nucleus or the centrosome and cell centroid was calculated (Figure 1C). The distribution of the angle between the centrosome-nucleus axis and the radius passing through the centrosome was also measured (Figure 1D). The typical intracellular organization of the cell in each condition is depicted (Figure 1B). Pan-cadherin (green), pericentrin (red), Hoechst (blue). Isolated cells (circle). Primary rat astrocytes were plated onto fibronectin-printed micropatterns and the distances between the nucleus or the centrosome and cell centroid was calculated (Figure 1C). The distribution of the angle between the centrosome-nucleus axis and the radius passing through the centrosome was also measured (Figure 1D). The typical intracellular organization of the cell in each condition is depicted (Figure 1B). Pan-cadherin (green), pericentrin (red), Hoechst (blue).U373 astrocytoma cells were submitted to a wound healing assay. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2E). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2F). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2D).Astrocytes were plated onto fibronectin-printed micropatterns and individual cells were enucleated followed by treatment with nocodazole. The distances between the centrosome and the cell centroids were measured (Figure 4E). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue) as shown in Figure 4D.Astrocytes were plated onto fibronectin-printed micropatterns and treated with cytochalasin D and nocodazole. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 4B). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 4C). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue) as shown in Figure 4A.Grouped cells with free edge. Primary rat astrocytes were plated onto fibronectin-printed micropatterns and the distances between the nucleus or the centrosome and cell centroid was calculated (Figure 1C). The distribution of the angle between the centrosome-nucleus axis and the radius passing through the centrosome was also measured (Figure 1D). The typical intracellular organization of the cell in each condition is depicted (Figure 1B). Pan-cadherin (green), pericentrin (red), Hoechst (blue). Astrocytes were plated onto U-shaped micropatterns coated with N-cadherin-Fc. The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome (Figure 3C) and the Y nucleus position (Figure 3D) was measured as depicted in Figure 3B. Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue) as shown in Figure 3A.Isolated cells (quadrant). Primary rat astrocytes were plated onto fibronectin-printed micropatterns and the distances between the nucleus or the centrosome and cell centroid was calculated (Figure 1C). The distribution of the angle between the centrosome-nucleus axis and the radius passing through the centrosome was also measured (Figure 1D). The typical intracellular organization of the cell in each condition is depicted (Figure 1B). Pan-cadherin (green), pericentrin (red), Hoechst (blue). Astrocytes were plated onto fibronectin printed micropatterns following nucleofection. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2E). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2F). The typical intracellular organization of the cell in each condition is depicted (Figure 2D, 2G). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2D).Astrocytes were plated onto circle-shaped micropatterns coated with N-cadherin-Fc. The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome (Figure 3C) and the Y nucleus position (Figure 3D) was measured as depicted in Figure 3B. Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue) as shown in Figure 3A.Astrocytes were plated onto fibronectin-printed micropatterns and treated with nocodazole. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 4B). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 4C). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue) as shown in Figure 4A.Astrocytes were plated onto fibronectin printed micropatterns following nucleofection with si N-cad1 and E-cad-GFP. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2E). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2F). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2D).Astrocytes were plated onto fibronectin printed micropatterns following nucleofection with si N-cad1. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2E). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2F). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2D).Astrocytes were plated onto fibronectin printed micropatterns following nucleofection with si N-cad1 and GFP-N-cad. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2E). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2F). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2D).Astrocytes were plated onto fibronectin printed micropatterns. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2E). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2F). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2D).Astrocytes were plated onto fibronectin-printed micropatterns and individual cells were enucleated. The distances between the centrosome and the cell centroids were measured (Figure 4E). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue) as shown in Figure 4D.Astrocytes were plated onto fibronectin printed micropatterns in the presence of calcium and were subsequently incubated for 5h in calcium-free medium. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 2B). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 2C). The typical intracellular organization of the cell in each condition is depicted (Figure 2A). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue), (Figure 2A).Astrocytes were plated onto fibronectin-printed micropatterns and treated with cytochalasin D. The distances between the nucleus or the centrosome and the cell centroids were measured (Figure 4B). The distribution of the angle between the centrosome-nucleus axis and the micro-pattern radius passing through the centrosome was also measured (Figure 4C). Cells are stained for pan-cadherin or E-cadherin (green), pericentrin (red) and Hoechst (blue) as shown in Figure 4A.Migrating cells Primary rat astrocytes were submitted to a wound-healing assay and the distances between the nucleus or the centrosome and cell centroid was calculated (Figure 1C). The distribution of the angle between the centrosome-nucleus axis and the radius passing through the centrosome was also measured (Figure 1D). The typical intracellular organization of the cell in each condition is depicted (Figure 1B).bioimagesImage 2906 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3258 (Fig 3 - "U" N-cad-Fc)Image 2781 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2618 (Fig 2 - Figure 2 - si N-cad1)Image 2025 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2051 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3451 (Fig 4 - Figure 4 - CytoD)Image 2930 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3617 (Fig 4 - Figure 4 – Enucleation)Image 3091 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3628 (Fig 4 - Figure 4 – Enucleation)Image 1995 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2495 (Fig 2 - Figure 2 - si control)Image 3075 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2668 (Fig 2 - Figure 2 - U373)Image 2572 (Fig 2 - Figure 2 - si N-cad1)Image 2028 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3158 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2867 (Fig 2 - si N-cad1 (migration))Image 2190 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2568 (Fig 2 - Figure 2 - si N-cad1)Image 2476 (Fig 2 - Figure 2 - si control)Image 2622 (Fig 2 - Figure 2 - si N-cad1)Image 3592 (Fig 4 - Figure 4 – Enucleation)Image 1992 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2080 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2183 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2691 (Fig 2 - Figure 2 - U373)Image 3361 (Fig 4 - Figure 4 - CytoD)Image 2848 (Fig 2 - si N-cad1 (migration))Image 2911 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3317 (Fig 4 - Figure 4 - CytoD)Image 3180 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3710 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3240 (Fig 3 - "U" N-cad-Fc)Image 2675 (Fig 2 - Figure 2 - U373)Image 3606 (Fig 4 - Figure 4 – Enucleation)Image 1998 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2081 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2246 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2122 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2022 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3193 (Fig 3 - Figure 3 - Circle N-cad-Fc)Fig 2 - si N-cad1 (migration)Image 3056 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3472 (Fig 4 - Figure 4 - Noco)Image 3061 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3684 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2785 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2322 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3674 (Fig 4 - Figure 4 – Enucleation)Image 3336 (Fig 4 - Figure 4 - CytoD)Image 3018 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2570 (Fig 2 - Figure 2 - si N-cad1)Image 2899 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3582 (Fig 4 - Figure 4 – CytD + Noco)Image 2624 (Fig 2 - Figure 2 - si N-cad1)Image 2992 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2529 (Fig 2 - Figure 2 - si control)Image 3589 (Fig 4 - Figure 4 – CytD + Noco)Image 3746 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1986 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3571 (Fig 4 - Figure 4 – CytD + Noco)Image 2169 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3663 (Fig 4 - Figure 4 – Enucleation)Image 2267 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3023 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2822 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2019 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Fig 3 - "U" N-cad-FcImage 2200 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2481 (Fig 2 - Figure 2 - si control)Image 3560 (Fig 4 - Figure 4 – CytD + Noco)Image 2013 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2010 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3145 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2783 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2620 (Fig 2 - Figure 2 - si N-cad1)Image 2016 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2534 (Fig 2 - Figure 2 - si control)Image 3265 (Fig 3 - "U" N-cad-Fc)Image 2050 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2987 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2176 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3080 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3132 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2829 (Fig 2 - si N-cad1 (migration))Image 3728 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2263 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2326 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2925 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2834 (Fig 2 - si N-cad1 (migration))Image 1989 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3304 (Fig 4 - Figure 4 - CytoD)Image 3639 (Fig 4 - Figure 4 – Enucleation)Image 2977 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2820 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2982 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2737 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3005 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2540 (Fig 2 - Figure 2 - si control)Image 2520 (Fig 2 - Figure 2 - si control)Image 3284 (Fig 3 - "U" N-cad-Fc)Image 3610 (Fig 4 - Figure 4 – Enucleation)Image 2766 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3755 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3621 (Fig 4 - Figure 4 – Enucleation)Image 3536 (Fig 4 - Figure 4 – CytD + Noco)Image 2976 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3177 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3474 (Fig 4 - Figure 4 - Noco)Image 3161 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2225 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2840 (Fig 2 - si N-cad1 (migration))Image 2641 (Fig 2 - Figure 2 - U373)Image 3632 (Fig 4 - Figure 4 – Enucleation)Image 3456 (Fig 4 - Figure 4 - CytoD)Image 3295 (Fig 4 - Figure 4 - CytoD)Image 3525 (Fig 4 - Figure 4 - Noco)Image 2787 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2216 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3729 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2195 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2712 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3010 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2290 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3252 (Fig 3 - "U" N-cad-Fc)Image 3558 (Fig 4 - Figure 4 – CytD + Noco)Image 2958 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2280 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3499 (Fig 4 - Figure 4 - Noco)Image 3470 (Fig 4 - Figure 4 - Noco)Image 2309 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3312 (Fig 4 - Figure 4 - CytoD)Image 3328 (Fig 4 - Figure 4 - CytoD)Image 2616 (Fig 2 - Figure 2 - si N-cad1)Image 2682 (Fig 2 - Figure 2 - U373)Image 3151 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2515 (Fig 2 - Figure 2 - si control)Image 2756 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2564 (Fig 2 - Figure 2 - si N-cad1)Image 2805 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3509 (Fig 4 - Figure 4 - Noco)Image 2614 (Fig 2 - Figure 2 - si N-cad1)Image 3547 (Fig 4 - Figure 4 – CytD + Noco)Image 2666 (Fig 2 - Figure 2 - U373)Image 3126 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2963 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3042 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2684 (Fig 2 - Figure 2 - U373)Image 3529 (Fig 4 - Figure 4 - Noco)Image 2939 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2801 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2138 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2566 (Fig 2 - Figure 2 - si N-cad1)Image 2853 (Fig 2 - si N-cad1 (migration))Image 2049 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2043 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3676 (Fig 4 - Figure 4 – Enucleation)Image 2549 (Fig 2 - Figure 2 - si control)Image 2242 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2912 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2188 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2040 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3116 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3665 (Fig 4 - Figure 4 – Enucleation)Image 3355 (Fig 4 - Figure 4 - CytoD)Image 2944 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3527 (Fig 4 - Figure 4 - Noco)Image 2115 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2872 (Fig 2 - si N-cad1 (migration))Image 3310 (Fig 4 - Figure 4 - CytoD)Image 3643 (Fig 4 - Figure 4 – Enucleation)Image 2301 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3277 (Fig 3 - "U" N-cad-Fc)Image 2826 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3569 (Fig 4 - Figure 4 – CytD + Noco)Image 3037 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2716 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3331 (Fig 4 - Figure 4 - CytoD)Image 1939 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2232 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2298 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2686 (Fig 2 - Figure 2 - U373)Image 2501 (Fig 2 - Figure 2 - si control)Image 3599 (Fig 4 - Figure 4 – Enucleation)Image 3654 (Fig 4 - Figure 4 – Enucleation)Image 2890 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2108 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2885 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3608 (Fig 4 - Figure 4 – Enucleation)Image 3754 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3309 (Fig 4 - Figure 4 - CytoD)Image 2209 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2752 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2288 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3719 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3187 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2046 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2926 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3155 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3619 (Fig 4 - Figure 4 – Enucleation)Image 1968 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2599 (Fig 2 - Figure 2 - si N-cad1)Image 2950 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2100 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2253 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2211 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2740 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2612 (Fig 2 - Figure 2 - si N-cad1)Image 2480 (Fig 2 - Figure 2 - si control)Image 3055 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3306 (Fig 4 - Figure 4 - CytoD)Image 2475 (Fig 2 - Figure 2 - si control)Image 2139 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3439 (Fig 4 - Figure 4 - CytoD)Image 2332 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2750 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3736 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2333 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3540 (Fig 4 - Figure 4 – CytD + Noco)Image 3183 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3074 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2187 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2907 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3142 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2731 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2075 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2337 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3562 (Fig 4 - Figure 4 – CytD + Noco)Image 3017 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3060 (Fig 2 - si N-cad1 + E-cad-GFP)Image 1945 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2319 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2562 (Fig 2 - Figure 2 - si N-cad1)Image 1962 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3297 (Fig 4 - Figure 4 - CytoD)Image 3551 (Fig 4 - Figure 4 – CytD + Noco)Image 2849 (Fig 2 - si N-cad1 (migration))Image 3523 (Fig 4 - Figure 4 - Noco)Image 2945 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2160 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2721 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3516 (Fig 4 - Figure 4 - Noco)Image 2988 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2733 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 1954 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1892 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3022 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3672 (Fig 4 - Figure 4 – Enucleation)Image 2983 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3762 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3469 (Fig 4 - Figure 4 - Noco)Image 3292 (Fig 4 - Figure 4 - CytoD)Image 2119 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3135 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3271 (Fig 3 - "U" N-cad-Fc)Image 3584 (Fig 4 - Figure 4 – CytD + Noco)Image 2004 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Fig 4 - Figure 4 - NocoImage 3507 (Fig 4 - Figure 4 - Noco)Image 2126 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2671 (Fig 2 - Figure 2 - U373)Image 3069 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3122 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3661 (Fig 4 - Figure 4 – Enucleation)Image 2597 (Fig 2 - Figure 2 - si N-cad1)Image 3573 (Fig 4 - Figure 4 – CytD + Noco)Image 2993 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2153 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3141 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3349 (Fig 4 - Figure 4 - CytoD)Image 3756 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3003 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3245 (Fig 3 - "U" N-cad-Fc)Image 2560 (Fig 2 - Figure 2 - si N-cad1)Image 2610 (Fig 2 - Figure 2 - si N-cad1)Image 3467 (Fig 4 - Figure 4 - CytoD)Image 3490 (Fig 4 - Figure 4 - Noco)Image 2835 (Fig 2 - si N-cad1 (migration))Image 2146 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3347 (Fig 4 - Figure 4 - CytoD)Image 2133 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2931 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2204 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2978 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2217 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3691 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1974 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2636 (Fig 2 - Figure 2 - U373)Image 3650 (Fig 4 - Figure 4 – Enucleation)Image 3315 (Fig 4 - Figure 4 - CytoD)Image 2273 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3502 (Fig 4 - Figure 4 - Noco)Image 3711 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2535 (Fig 2 - Figure 2 - si control)Image 2873 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3739 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2789 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3532 (Fig 4 - Figure 4 - Noco)Image 2659 (Fig 2 - Figure 2 - U373)Image 2516 (Fig 2 - Figure 2 - si control)Image 3534 (Fig 4 - Figure 4 – CytD + Noco)Image 2199 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2305 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2714 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2817 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3149 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3623 (Fig 4 - Figure 4 – Enucleation)Image 2074 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2836 (Fig 2 - si N-cad1 (migration))Image 3712 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2841 (Fig 2 - si N-cad1 (migration))Fig 4 - Figure 4 – CytD + NocoImage 2554 (Fig 2 - Figure 2 - si N-cad1)Image 2604 (Fig 2 - Figure 2 - si N-cad1)Image 3257 (Fig 3 - "U" N-cad-Fc)Image 3464 (Fig 4 - Figure 4 - CytoD)Image 1953 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2758 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2886 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2704 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3634 (Fig 4 - Figure 4 – Enucleation)Image 2807 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3692 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2226 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3645 (Fig 4 - Figure 4 – Enucleation)Image 2739 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 1983 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3125 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3567 (Fig 4 - Figure 4 – CytD + Noco)Image 3139 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2274 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2746 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2521 (Fig 2 - Figure 2 - si control)Image 3481 (Fig 4 - Figure 4 - Noco)Image 3738 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2287 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3301 (Fig 4 - Figure 4 - CytoD)Image 3578 (Fig 4 - Figure 4 – CytD + Noco)Image 3656 (Fig 4 - Figure 4 – Enucleation)Image 3761 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2854 (Fig 2 - si N-cad1 (migration))Image 3004 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2312 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2127 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3340 (Fig 4 - Figure 4 - CytoD)Image 2824 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3597 (Fig 4 - Figure 4 – Enucleation)Image 2079 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2294 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2212 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2959 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3342 (Fig 4 - Figure 4 - CytoD)Image 3129 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3693 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3678 (Fig 4 - Figure 4 – Enucleation)Image 2913 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2205 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3290 (Fig 4 - Figure 4 - CytoD)Image 2964 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3667 (Fig 4 - Figure 4 – Enucleation)Image 3196 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2548 (Fig 2 - Figure 2 - si control)Image 3344 (Fig 4 - Figure 4 - CytoD)Image 2076 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2034 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2134 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2502 (Fig 2 - Figure 2 - si control)Image 3264 (Fig 3 - "U" N-cad-Fc)Image 2606 (Fig 2 - Figure 2 - si N-cad1)Image 2556 (Fig 2 - Figure 2 - si N-cad1)Image 3201 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3319 (Fig 4 - Figure 4 - CytoD)Image 2121 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2891 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2494 (Fig 2 - Figure 2 - si control)Image 3462 (Fig 4 - Figure 4 - CytoD)Image 3119 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2868 (Fig 2 - si N-cad1 (migration))Image 3737 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2239 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2764 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 1959 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2078 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3556 (Fig 4 - Figure 4 – CytD + Noco)Image 2558 (Fig 2 - Figure 2 - si N-cad1)Image 2908 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2608 (Fig 2 - Figure 2 - si N-cad1)Image 2679 (Fig 2 - Figure 2 - U373)Image 2194 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2077 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2141 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3694 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3041 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2489 (Fig 2 - Figure 2 - si control)Image 2695 (Fig 2 - Figure 2 - U373)Image 3036 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3545 (Fig 4 - Figure 4 – CytD + Noco)Image 3466 (Fig 4 - Figure 4 - CytoD)Image 2008 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2792 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3035 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2247 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3494 (Fig 4 - Figure 4 - Noco)Image 2970 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3364 (Fig 4 - Figure 4 - CytoD)Image 3624 (Fig 4 - Figure 4 – Enucleation)Image 2639 (Fig 2 - Figure 2 - U373)Image 2846 (Fig 2 - si N-cad1 (migration))Image 2851 (Fig 2 - si N-cad1 (migration))Image 2163 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3726 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2135 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2793 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 1919 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 1972 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1897 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2048 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3333 (Fig 4 - Figure 4 - CytoD)Image 2005 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Fig 2 - si N-cad1 + GFP-N-cadImage 2149 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2927 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2249 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3596 (Fig 4 - Figure 4 – Enucleation)Image 2325 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2474 (Fig 2 - Figure 2 - si control)Image 2177 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2709 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2497 (Fig 2 - Figure 2 - si control)Image 2262 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3454 (Fig 4 - Figure 4 - CytoD)Image 3302 (Fig 4 - Figure 4 - CytoD)Image 2551 (Fig 2 - Figure 2 - si N-cad1)Image 3345 (Fig 4 - Figure 4 - CytoD)Image 3602 (Fig 4 - Figure 4 – Enucleation)Image 2057 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2208 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3730 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2504 (Fig 2 - Figure 2 - si control)Image 2989 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3660 (Fig 4 - Figure 4 – Enucleation)Image 2527 (Fig 2 - Figure 2 - si control)Image 2994 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3073 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3251 (Fig 3 - "U" N-cad-Fc)Image 3668 (Fig 4 - Figure 4 – Enucleation)Image 2601 (Fig 2 - Figure 2 - si N-cad1)Image 3247 (Fig 3 - "U" N-cad-Fc)Image 2589 (Fig 2 - Figure 2 - si N-cad1)Image 2532 (Fig 2 - Figure 2 - si control)Image 3709 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2086 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2264 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2039 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3068 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3254 (Fig 3 - "U" N-cad-Fc)Image 3110 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3495 (Fig 4 - Figure 4 - Noco)Image 2587 (Fig 2 - Figure 2 - si N-cad1)Image 3138 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2729 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2107 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3002 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3511 (Fig 4 - Figure 4 - Noco)Image 1896 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3157 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2293 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2323 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2266 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2708 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2797 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).Image 2813 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2904 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2542 (Fig 2 - Figure 2 - si control)Image 1927 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2932 (Fig 2 - si N-cad1 + GFP-N-cad)Image 1918 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3505 (Fig 4 - Figure 4 - Noco)Fig 2 - Figure 2 - si controlImage 3520 (Fig 4 - Figure 4 - Noco)Image 2874 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3503 (Fig 4 - Figure 4 - Noco)Image 2645 (Fig 2 - Figure 2 - U373)Image 2654 (Fig 2 - Figure 2 - U373)Image 3159 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2189 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3757 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3552 (Fig 4 - Figure 4 – CytD + Noco)Image 2592 (Fig 2 - Figure 2 - si N-cad1)Image 2646 (Fig 2 - Figure 2 - U373)Image 2897 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3488 (Fig 4 - Figure 4 - Noco)Image 2660 (Fig 2 - Figure 2 - U373)Image 2072 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 1969 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3131 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2036 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2594 (Fig 2 - Figure 2 - si N-cad1)Image 3146 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2652 (Fig 2 - Figure 2 - U373)Image 3549 (Fig 4 - Figure 4 – CytD + Noco)Image 2772 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3486 (Fig 4 - Figure 4 - Noco)Image 3308 (Fig 4 - Figure 4 - CytoD)Image 2069 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2748 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3358 (Fig 4 - Figure 4 - CytoD)Image 2777 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Fig 2 - Figure 2 - si N-cad1Image 2123 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3241 (Fig 3 - "U" N-cad-Fc)Image 2220 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3601 (Fig 4 - Figure 4 – Enucleation)Image 2517 (Fig 2 - Figure 2 - si control)Image 3327 (Fig 4 - Figure 4 - CytoD)Image 3136 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2585 (Fig 2 - Figure 2 - si N-cad1)Image 2206 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2590 (Fig 2 - Figure 2 - si N-cad1)Image 2648 (Fig 2 - Figure 2 - U373)Image 2306 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3448 (Fig 4 - Figure 4 - CytoD)Image 3718 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3128 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3554 (Fig 4 - Figure 4 – CytD + Noco)Image 2727 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2887 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3095 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3687 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1960 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3167 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3574 (Fig 4 - Figure 4 – CytD + Noco)Image 2175 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2140 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2165 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2702 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2112 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2235 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2909 (Fig 2 - si N-cad1 + GFP-N-cad)Image 1929 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3058 (Fig 2 - si N-cad1 + E-cad-GFP)Image 1944 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3743 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2011 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3288 (Fig 3 - "U" N-cad-Fc)Image 2812 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3181 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2892 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2773 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Fig 4 - Figure 4 - CytoDImage 3012 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3352 (Fig 4 - Figure 4 - CytoD)Image 2723 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3063 (Fig 2 - si N-cad1 + E-cad-GFP)Fig 3 - Figure 3 - Circle N-cad-FcFig 4 - Figure 4 – Enucleation + NocoImage 1987 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2522 (Fig 2 - Figure 2 - si control)Image 1908 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3197 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 1935 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3646 (Fig 4 - Figure 4 – Enucleation)Image 2090 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2688 (Fig 2 - Figure 2 - U373)Image 1938 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3298 (Fig 4 - Figure 4 - CytoD)Image 2798 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3171 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3007 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2088 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2819 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3040 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3244 (Fig 3 - "U" N-cad-Fc)Image 3199 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3720 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3681 (Fig 4 - Figure 4 – Enucleation)Image 2869 (Fig 2 - si N-cad1 (migration))Image 1941 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1984 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2278 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2311 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2547 (Fig 2 - Figure 2 - si control)Image 2321 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2965 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2222 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3106 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2914 (Fig 2 - si N-cad1 + GFP-N-cad)Fig 4 - Figure 4 – EnucleationImage 2942 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2937 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2791 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 1985 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2550 (Fig 2 - Figure 2 - si control)Image 2334 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3483 (Fig 4 - Figure 4 - Noco)Image 3015 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3747 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2578 (Fig 2 - Figure 2 - si N-cad1)Image 3094 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3508 (Fig 4 - Figure 4 - Noco)Image 3626 (Fig 4 - Figure 4 – Enucleation)Image 2694 (Fig 2 - Figure 2 - U373)Image 2111 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2871 (Fig 2 - si N-cad1 (migration))Image 2866 (Fig 2 - si N-cad1 (migration))Image 3458 (Fig 4 - Figure 4 - CytoD)Image 2633 (Fig 2 - Figure 2 - si N-cad1)Image 3170 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3368 (Fig 4 - Figure 4 - CytoD)Image 2251 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2038 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2545 (Fig 2 - Figure 2 - si control)Image 3548 (Fig 4 - Figure 4 – CytD + Noco)Image 2125 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2741 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2252 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3594 (Fig 4 - Figure 4 – Enucleation)Image 2947 (Fig 2 - si N-cad1 + GFP-N-cad)Image 1942 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2894 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2680 (Fig 2 - Figure 2 - U373)Image 3648 (Fig 4 - Figure 4 – Enucleation)Image 3100 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2152 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3020 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3191 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3753 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1905 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2657 (Fig 2 - Figure 2 - U373)Image 3285 (Fig 3 - "U" N-cad-Fc)Image 3168 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2236 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2335 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2628 (Fig 2 - Figure 2 - si N-cad1)Image 3127 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2985 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3531 (Fig 4 - Figure 4 - Noco)Image 2059 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2919 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2084 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2289 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3350 (Fig 4 - Figure 4 - CytoD)Image 1971 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2722 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3048 (Fig 2 - si N-cad1 + E-cad-GFP)Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.Image 2710 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2889 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2833 (Fig 2 - si N-cad1 (migration))Image 2509 (Fig 2 - Figure 2 - si control)Image 3190 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3500 (Fig 4 - Figure 4 - Noco)Image 3685 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2110 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1933 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3722 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2096 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3680 (Fig 4 - Figure 4 – Enucleation)Image 2514 (Fig 2 - Figure 2 - si control)Image 2210 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1957 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2024 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2924 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3669 (Fig 4 - Figure 4 – Enucleation)Image 2635 (Fig 2 - Figure 2 - si N-cad1)Image 2952 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3497 (Fig 4 - Figure 4 - Noco)Image 2735 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3282 (Fig 3 - "U" N-cad-Fc)Image 2828 (Fig 2 - si N-cad1 (migration))Image 3120 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3647 (Fig 4 - Figure 4 – Enucleation)Image 2477 (Fig 2 - Figure 2 - si control)Image 3745 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3325 (Fig 4 - Figure 4 - CytoD)Image 2901 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3030 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2250 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2643 (Fig 2 - Figure 2 - U373)Image 3148 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3360 (Fig 4 - Figure 4 - CytoD)Image 2742 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3477 (Fig 4 - Figure 4 - Noco)Image 2583 (Fig 2 - Figure 2 - si N-cad1)Image 3278 (Fig 3 - "U" N-cad-Fc)Image 3604 (Fig 4 - Figure 4 – Enucleation)Image 2991 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2581 (Fig 2 - Figure 2 - si N-cad1)Image 3492 (Fig 4 - Figure 4 - Noco)Image 3076 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3147 (Fig 3 - Figure 3 - Circle N-cad-Fc)Fig 2 - Figure 2 - Astroctyes (-) CalciumImage 2482 (Fig 2 - Figure 2 - si control)Image 3053 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3576 (Fig 4 - Figure 4 – CytD + Noco)Image 3446 (Fig 4 - Figure 4 - CytoD)Image 3751 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3705 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2818 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2637 (Fig 2 - Figure 2 - U373)Image 2336 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3337 (Fig 4 - Figure 4 - CytoD)Image 3595 (Fig 4 - Figure 4 – Enucleation)Image 2237 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3081 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2631 (Fig 2 - Figure 2 - si N-cad1)Image 2223 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3025 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3130 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3275 (Fig 3 - "U" N-cad-Fc)Image 2980 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3625 (Fig 4 - Figure 4 – Enucleation)Image 2277 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2310 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2856 (Fig 2 - si N-cad1 (migration))Image 2703 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2861 (Fig 2 - si N-cad1 (migration))Image 2806 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3603 (Fig 4 - Figure 4 – Enucleation)Image 3066 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3630 (Fig 4 - Figure 4 – Enucleation)Image 2082 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3198 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2224 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 1943 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2537 (Fig 2 - Figure 2 - si control)Image 3528 (Fig 4 - Figure 4 - Noco)Image 1899 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3268 (Fig 3 - "U" N-cad-Fc)Image 2975 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2663 (Fig 2 - Figure 2 - U373)Image 3366 (Fig 4 - Figure 4 - CytoD)Image 2778 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2276 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2574 (Fig 2 - Figure 2 - si N-cad1)Image 3514 (Fig 4 - Figure 4 - Noco)Image 2109 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3140 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3749 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2487 (Fig 2 - Figure 2 - si control)Image 2037 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2838 (Fig 2 - si N-cad1 (migration))Image 2098 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3339 (Fig 4 - Figure 4 - CytoD)Image 3323 (Fig 4 - Figure 4 - CytoD)Image 3724 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2007 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2576 (Fig 2 - Figure 2 - si N-cad1)Image 2626 (Fig 2 - Figure 2 - si N-cad1)Image 3701 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2934 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3362 (Fig 4 - Figure 4 - CytoD)Image 3652 (Fig 4 - Figure 4 – Enucleation)Image 2747 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2962 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2728 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2957 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2137 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3321 (Fig 4 - Figure 4 - CytoD)Image 3675 (Fig 4 - Figure 4 – Enucleation)Image 1999 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2124 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2929 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3038 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2879 (Fig 2 - si N-cad1 + GFP-N-cad)Image 1894 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2843 (Fig 2 - si N-cad1 (migration))Image 3150 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2191 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2053 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2492 (Fig 2 - Figure 2 - si control)Image 2999 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3178 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3575 (Fig 4 - Figure 4 – CytD + Noco)Image 3043 (Fig 2 - si N-cad1 + E-cad-GFP)Image 1916 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3442 (Fig 4 - Figure 4 - CytoD)Image 3580 (Fig 4 - Figure 4 – CytD + Noco)Image 3117 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3272 (Fig 3 - "U" N-cad-Fc)Image 2754 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3071 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3703 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2677 (Fig 2 - Figure 2 - U373)Image 2291 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2055 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2884 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3440 (Fig 4 - Figure 4 - CytoD)Image 2308 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 1956 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2023 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2151 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2164 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2761 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3553 (Fig 4 - Figure 4 – CytD + Noco)Image 1922 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2697 (Fig 2 - Figure 2 - U373)Image 3160 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3683 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3188 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3107 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2506 (Fig 2 - Figure 2 - si control)Image 2530 (Fig 2 - Figure 2 - si control)Image 2094 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2328 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3519 (Fig 4 - Figure 4 - Noco)Image 3620 (Fig 4 - Figure 4 – Enucleation)Image 3200 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3329 (Fig 4 - Figure 4 - CytoD)Image 1930 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2683 (Fig 2 - Figure 2 - U373)Image 3535 (Fig 4 - Figure 4 – CytD + Noco)Image 2986 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3124 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2341 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3689 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2780 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3330 (Fig 4 - Figure 4 - CytoD)Image 2567 (Fig 2 - Figure 2 - si N-cad1)Image 2895 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3137 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2248 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3014 (Fig 2 - si N-cad1 + E-cad-GFP)Image 1958 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2197 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2996 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2967 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2525 (Fig 2 - Figure 2 - si control)Image 3546 (Fig 4 - Figure 4 – CytD + Noco)Image 2623 (Fig 2 - Figure 2 - si N-cad1)Image 2221 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1921 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2881 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3741 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3480 (Fig 4 - Figure 4 - Noco)Image 2676 (Fig 2 - Figure 2 - U373)Image 3526 (Fig 4 - Figure 4 - Noco)Image 1952 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2617 (Fig 2 - Figure 2 - si N-cad1)Image 2244 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2129 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2544 (Fig 2 - Figure 2 - si control)Image 2690 (Fig 2 - Figure 2 - U373)Image 3179 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 1955 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3262 (Fig 3 - "U" N-cad-Fc)Image 3367 (Fig 4 - Figure 4 - CytoD)Image 3033 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3296 (Fig 4 - Figure 4 - CytoD)Image 3664 (Fig 4 - Figure 4 – Enucleation)Image 3111 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2830 (Fig 2 - si N-cad1 (migration))Image 2876 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2719 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3166 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 1901 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3715 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3438 (Fig 4 - Figure 4 - CytoD)Image 3052 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3444 (Fig 4 - Figure 4 - CytoD)Image 2953 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2143 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3698 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3473 (Fig 4 - Figure 4 - Noco)Image 2640 (Fig 2 - Figure 2 - U373)Image 2207 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2136 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3581 (Fig 4 - Figure 4 – CytD + Noco)Image 2621 (Fig 2 - Figure 2 - si N-cad1)Image 2825 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3579 (Fig 4 - Figure 4 – CytD + Noco)Image 2800 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3305 (Fig 4 - Figure 4 - CytoD)Image 2863 (Fig 2 - si N-cad1 (migration))Image 2573 (Fig 2 - Figure 2 - si N-cad1)Image 2162 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2717 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3287 (Fig 3 - "U" N-cad-Fc)Image 3653 (Fig 4 - Figure 4 – Enucleation)Image 2499 (Fig 2 - Figure 2 - si control)Image 1949 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1920 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3348 (Fig 4 - Figure 4 - CytoD)Image 2858 (Fig 2 - si N-cad1 (migration))Image 3568 (Fig 4 - Figure 4 – CytD + Noco)Image 2948 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3000 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3047 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2571 (Fig 2 - Figure 2 - si N-cad1)Image 3557 (Fig 4 - Figure 4 – CytD + Noco)Image 3642 (Fig 4 - Figure 4 – Enucleation)Image 2214 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2511 (Fig 2 - Figure 2 - si control)Image 2902 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3631 (Fig 4 - Figure 4 – Enucleation)Image 2802 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2113 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2227 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2296 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3457 (Fig 4 - Figure 4 - CytoD)Image 1902 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2763 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 1946 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2095 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 1911 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2240 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3243 (Fig 3 - "U" N-cad-Fc)Image 2715 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2282 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2916 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3195 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2615 (Fig 2 - Figure 2 - si N-cad1)Image 2940 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3616 (Fig 4 - Figure 4 – Enucleation)Image 2759 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3627 (Fig 4 - Figure 4 – Enucleation)Image 3118 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3335 (Fig 4 - Figure 4 - CytoD)Image 3065 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2192 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3605 (Fig 4 - Figure 4 – Enucleation)Image 2275 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2788 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 1932 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2000 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3143 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2935 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2765 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3707 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2713 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2692 (Fig 2 - Figure 2 - U373)Image 2178 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3046 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3638 (Fig 4 - Figure 4 – Enucleation)Image 1973 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2491 (Fig 2 - Figure 2 - si control)Image 3733 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3649 (Fig 4 - Figure 4 – Enucleation)Image 2565 (Fig 2 - Figure 2 - si N-cad1)Image 1903 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2921 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3706 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1923 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3248 (Fig 3 - "U" N-cad-Fc)Image 2093 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2292 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2804 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2307 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2120 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2092 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2738 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2185 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3079 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2563 (Fig 2 - Figure 2 - si N-cad1)Image 2063 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 1970 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3517 (Fig 4 - Figure 4 - Noco)Image 3570 (Fig 4 - Figure 4 – CytD + Noco)Image 3153 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2674 (Fig 2 - Figure 2 - U373)Image 3084 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2538 (Fig 2 - Figure 2 - si control)Image 2486 (Fig 2 - Figure 2 - si control)Image 2150 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3093 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3593 (Fig 4 - Figure 4 – Enucleation)Image 3449 (Fig 4 - Figure 4 - CytoD)Image 2699 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2219 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2569 (Fig 2 - Figure 2 - si N-cad1)Image 3673 (Fig 4 - Figure 4 – Enucleation)Image 2155 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1912 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3028 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3533 (Fig 4 - Figure 4 - Noco)Image 3588 (Fig 4 - Figure 4 – CytD + Noco)Image 2064 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2265 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2823 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2519 (Fig 2 - Figure 2 - si control)Image 3450 (Fig 4 - Figure 4 - CytoD)Image 2148 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2784 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3732 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2067 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3577 (Fig 4 - Figure 4 – CytD + Noco)Image 2839 (Fig 2 - si N-cad1 (migration))Image 3009 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2314 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2009 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2844 (Fig 2 - si N-cad1 (migration))Image 3311 (Fig 4 - Figure 4 - CytoD)Image 2769 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2258 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3651 (Fig 4 - Figure 4 – Enucleation)Image 2619 (Fig 2 - Figure 2 - si N-cad1)Image 2324 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2972 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3688 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2065 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3185 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3354 (Fig 4 - Figure 4 - CytoD)Image 1976 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2678 (Fig 2 - Figure 2 - U373)Image 2006 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3070 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2331 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3108 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2808 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2734 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 1979 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1900 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3281 (Fig 3 - "U" N-cad-Fc)Image 2524 (Fig 2 - Figure 2 - si control)Image 3255 (Fig 3 - "U" N-cad-Fc)Image 3662 (Fig 4 - Figure 4 – Enucleation)Image 2066 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2003 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3452 (Fig 4 - Figure 4 - CytoD)Image 2180 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2526 (Fig 2 - Figure 2 - si control)Image 2089 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2557 (Fig 2 - Figure 2 - si N-cad1)Image 2561 (Fig 2 - Figure 2 - si N-cad1)Image 3611 (Fig 4 - Figure 4 – Enucleation)Image 2672 (Fig 2 - Figure 2 - U373)Image 1988 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2018 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2173 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3622 (Fig 4 - Figure 4 – Enucleation)Image 3267 (Fig 3 - "U" N-cad-Fc)Image 2035 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3189 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3242 (Fig 3 - "U" N-cad-Fc)Image 3699 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3341 (Fig 4 - Figure 4 - CytoD)Image 1924 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3742 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2607 (Fig 2 - Figure 2 - si N-cad1)Image 3544 (Fig 4 - Figure 4 – CytD + Noco)Image 3176 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 1982 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3121 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3598 (Fig 4 - Figure 4 – Enucleation)Image 2062 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2159 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3566 (Fig 4 - Figure 4 – CytD + Noco)Image 2070 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3644 (Fig 4 - Figure 4 – Enucleation)Image 2238 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2611 (Fig 2 - Figure 2 - si N-cad1)Image 3498 (Fig 4 - Figure 4 - Noco)Image 2665 (Fig 2 - Figure 2 - U373)Image 3318 (Fig 4 - Figure 4 - CytoD)Image 2968 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3555 (Fig 4 - Figure 4 – CytD + Noco)Image 2531 (Fig 2 - Figure 2 - si control)Image 2877 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2498 (Fig 2 - Figure 2 - si control)Image 2254 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2730 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2831 (Fig 2 - si N-cad1 (migration))Image 3356 (Fig 4 - Figure 4 - CytoD)Image 2613 (Fig 2 - Figure 2 - si N-cad1)Image 2012 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2218 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3274 (Fig 3 - "U" N-cad-Fc)Image 3758 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3633 (Fig 4 - Figure 4 – Enucleation)Image 2543 (Fig 2 - Figure 2 - si control)Image 3032 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2651 (Fig 2 - Figure 2 - U373)Image 3359 (Fig 4 - Figure 4 - CytoD)Image 2949 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2061 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2193 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2649 (Fig 2 - Figure 2 - U373)Image 3078 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2317 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2755 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3522 (Fig 4 - Figure 4 - Noco)Image 3716 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2186 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3484 (Fig 4 - Figure 4 - Noco)Image 3677 (Fig 4 - Figure 4 – Enucleation)Image 2903 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3169 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3313 (Fig 4 - Figure 4 - CytoD)Image 1914 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2179 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1991 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2859 (Fig 2 - si N-cad1 (migration))Image 2021 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2642 (Fig 2 - Figure 2 - U373)Image 3083 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3655 (Fig 4 - Figure 4 – Enucleation)Image 2864 (Fig 2 - si N-cad1 (migration))Image 2233 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3489 (Fig 4 - Figure 4 - Noco)Image 2300 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3156 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2234 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2760 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3475 (Fig 4 - Figure 4 - Noco)Image 3696 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2479 (Fig 2 - Figure 2 - si control)Image 2339 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3051 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2598 (Fig 2 - Figure 2 - si N-cad1)Image 1997 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2484 (Fig 2 - Figure 2 - si control)Image 2313 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2724 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3303 (Fig 4 - Figure 4 - CytoD)Image 3491 (Fig 4 - Figure 4 - Noco)Image 2041 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3740 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2286 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2753 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 1906 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2779 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3697 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2762 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2507 (Fig 2 - Figure 2 - si control)Image 2954 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3468 (Fig 4 - Figure 4 - CytoD)Image 3734 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2166 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3294 (Fig 4 - Figure 4 - CytoD)Image 3666 (Fig 4 - Figure 4 – Enucleation)Fig 4Image 2512 (Fig 2 - Figure 2 - si control)Image 2896 (Fig 2 - si N-cad1 + GFP-N-cad)Fig 3Fig 2Fig 1Fig 2 - Figure 2 - Astrocytes (+) CalciumImage 3343 (Fig 4 - Figure 4 - CytoD)Image 3115 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2106 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3045 (Fig 2 - si N-cad1 + E-cad-GFP)Image 1913 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2161 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2936 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3618 (Fig 4 - Figure 4 – Enucleation)Image 2960 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2596 (Fig 2 - Figure 2 - si N-cad1)Image 1940 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2490 (Fig 2 - Figure 2 - si control)Image 3629 (Fig 4 - Figure 4 – Enucleation)Image 2485 (Fig 2 - Figure 2 - si control)Image 2736 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3182 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2154 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3316 (Fig 4 - Figure 4 - CytoD)Image 3506 (Fig 4 - Figure 4 - Noco)Image 2955 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3607 (Fig 4 - Figure 4 – Enucleation)Image 1907 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2685 (Fig 2 - Figure 2 - U373)Image 1936 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3261 (Fig 3 - "U" N-cad-Fc)Image 1990 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2605 (Fig 2 - Figure 2 - si N-cad1)Image 2553 (Fig 2 - Figure 2 - si N-cad1)Image 3027 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3286 (Fig 3 - "U" N-cad-Fc)Image 3050 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3461 (Fig 4 - Figure 4 - CytoD)Image 3346 (Fig 4 - Figure 4 - CytoD)Image 2047 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3550 (Fig 4 - Figure 4 – CytD + Noco)Image 2320 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2786 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3092 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2922 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3192 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3471 (Fig 4 - Figure 4 - Noco)Image 3460 (Fig 4 - Figure 4 - CytoD)Image 2814 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2147 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2711 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2767 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2681 (Fig 2 - Figure 2 - U373)Image 2279 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2917 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3291 (Fig 4 - Figure 4 - CytoD)Image 2259 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2174 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2973 (Fig 2 - si N-cad1 + GFP-N-cad)Image 1961 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3279 (Fig 3 - "U" N-cad-Fc)Image 2609 (Fig 2 - Figure 2 - si N-cad1)Image 2707 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2796 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3008 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3096 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3465 (Fig 4 - Figure 4 - CytoD)Image 3583 (Fig 4 - Figure 4 – CytD + Noco)Image 2603 (Fig 2 - Figure 2 - si N-cad1)Image 2167 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3759 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2882 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2241 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2539 (Fig 2 - Figure 2 - si control)Image 1931 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 1925 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 1967 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1996 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2555 (Fig 2 - Figure 2 - si N-cad1)Image 3300 (Fig 4 - Figure 4 - CytoD)Image 2669 (Fig 2 - Figure 2 - U373)Image 3101 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3713 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2181 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3162 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2020 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3105 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2261 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2845 (Fig 2 - si N-cad1 (migration))Image 3561 (Fig 4 - Figure 4 – CytD + Noco)Image 3760 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3086 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2338 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2689 (Fig 2 - Figure 2 - U373)Image 2941 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2997 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2771 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2821 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3714 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2101 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3059 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2318 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3172 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2026 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3600 (Fig 4 - Figure 4 – Enucleation)Image 2559 (Fig 2 - Figure 2 - si N-cad1)Image 2281 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3572 (Fig 4 - Figure 4 – CytD + Noco)Image 2850 (Fig 2 - si N-cad1 (migration))Image 3186 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3013 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3064 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3463 (Fig 4 - Figure 4 - CytoD)Image 3591 (Fig 4 - Figure 4 – Enucleation)Image 2743 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2546 (Fig 2 - Figure 2 - si control)Image 2946 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2770 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3314 (Fig 4 - Figure 4 - CytoD)Image 1928 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3109 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2329 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3613 (Fig 4 - Figure 4 – Enucleation)Image 2142 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2661 (Fig 2 - Figure 2 - U373)Image 2340 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 1978 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3539 (Fig 4 - Figure 4 – CytD + Noco)Image 3293 (Fig 4 - Figure 4 - CytoD)Image 3704 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1909 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3276 (Fig 3 - "U" N-cad-Fc)Image 3283 (Fig 3 - "U" N-cad-Fc)Image 2260 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2201 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3512 (Fig 4 - Figure 4 - Noco)Image 2299 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3054 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3752 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2827 (Fig 2 - si N-cad1 (migration))Image 3542 (Fig 4 - Figure 4 – CytD + Noco)Image 2215 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2342 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3097 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3721 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2865 (Fig 2 - si N-cad1 (migration))Image 2602 (Fig 2 - Figure 2 - si N-cad1)Image 3165 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2794 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3194 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 1937 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2951 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2114 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3269 (Fig 3 - "U" N-cad-Fc)Image 3695 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2170 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2002 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2045 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2588 (Fig 2 - Figure 2 - si N-cad1)Image 2600 (Fig 2 - Figure 2 - si N-cad1)Image 2795 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2552 (Fig 2 - Figure 2 - si N-cad1)Image 1975 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2042 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2870 (Fig 2 - si N-cad1 (migration))Image 3016 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2327 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2297 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3021 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3690 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2893 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3152 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2513 (Fig 2 - Figure 2 - si control)Image 3082 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2662 (Fig 2 - Figure 2 - U373)Image 2638 (Fig 2 - Figure 2 - U373)Image 2979 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2591 (Fig 2 - Figure 2 - si N-cad1)Image 3459 (Fig 4 - Figure 4 - CytoD)Image 3671 (Fig 4 - Figure 4 – Enucleation)Image 3735 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2969 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3357 (Fig 4 - Figure 4 - CytoD)Image 3280 (Fig 3 - "U" N-cad-Fc)Image 3679 (Fig 4 - Figure 4 – Enucleation)Image 3144 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2923 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3049 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2984 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2073 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3123 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2647 (Fig 2 - Figure 2 - U373)Image 2508 (Fig 2 - Figure 2 - si control)Image 3338 (Fig 4 - Figure 4 - CytoD)Image 2030 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3513 (Fig 4 - Figure 4 - Noco)Image 3031 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2243 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2774 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2655 (Fig 2 - Figure 2 - U373)Image 2832 (Fig 2 - si N-cad1 (migration))Image 2595 (Fig 2 - Figure 2 - si N-cad1)Image 2196 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1966 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2060 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2990 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3077 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3487 (Fig 4 - Figure 4 - Noco)Image 2116 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3369 (Fig 4 - Figure 4 - CytoD)Image 3504 (Fig 4 - Figure 4 - Noco)Image 3447 (Fig 4 - Figure 4 - CytoD)Image 2033 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2790 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2593 (Fig 2 - Figure 2 - si N-cad1)Image 2478 (Fig 2 - Figure 2 - si control)Image 2653 (Fig 2 - Figure 2 - U373)Image 3485 (Fig 4 - Figure 4 - Noco)Image 2799 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2706 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2483 (Fig 2 - Figure 2 - si control)Image 3478 (Fig 4 - Figure 4 - Noco)Image 2811 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3541 (Fig 4 - Figure 4 – CytD + Noco)Image 3493 (Fig 4 - Figure 4 - Noco)Image 3479 (Fig 4 - Figure 4 - Noco)Image 2156 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3326 (Fig 4 - Figure 4 - CytoD)Image 3686 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2168 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2956 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2304 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2536 (Fig 2 - Figure 2 - si control)Image 2726 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2130 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3521 (Fig 4 - Figure 4 - Noco)Image 3612 (Fig 4 - Figure 4 – Enucleation)Image 2283 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2900 (Fig 2 - si N-cad1 + GFP-N-cad)Image 1895 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3538 (Fig 4 - Figure 4 – CytD + Noco)Image 1917 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3102 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2749 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2182 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3453 (Fig 4 - Figure 4 - CytoD)Image 2670 (Fig 2 - Figure 2 - U373)Image 2860 (Fig 2 - si N-cad1 (migration))Image 2816 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2071 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3717 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2285 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 1963 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1926 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 3026 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2203 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3563 (Fig 4 - Figure 4 – CytD + Noco)Image 2027 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2586 (Fig 2 - Figure 2 - si N-cad1)Image 2855 (Fig 2 - si N-cad1 (migration))Image 2085 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2102 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2656 (Fig 2 - Figure 2 - U373)Image 2815 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2918 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3067 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2961 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3273 (Fig 3 - "U" N-cad-Fc)Image 2701 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3259 (Fig 3 - "U" N-cad-Fc)Image 3039 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3112 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2974 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2272 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3744 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3543 (Fig 4 - Figure 4 – CytD + Noco)Image 2213 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3044 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3351 (Fig 4 - Figure 4 - CytoD)Image 2837 (Fig 2 - si N-cad1 (migration))Image 2228 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2842 (Fig 2 - si N-cad1 (migration))Image 2017 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2776 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2503 (Fig 2 - Figure 2 - si control)Image 2998 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3072 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3175 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3307 (Fig 4 - Figure 4 - CytoD)Image 3670 (Fig 4 - Figure 4 – Enucleation)Image 2295 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3585 (Fig 4 - Figure 4 – CytD + Noco)Image 2493 (Fig 2 - Figure 2 - si control)Image 3708 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1981 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3099 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3104 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2198 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1947 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Fig 1 - Figure 1C and 1D - Isolated Cells (circle).Image 2488 (Fig 2 - Figure 2 - si control)Image 2014 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2087 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3657 (Fig 4 - Figure 4 – Enucleation)Image 3163 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2105 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 1898 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2745 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2878 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2883 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3332 (Fig 4 - Figure 4 - CytoD)Image 2268 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2128 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3270 (Fig 3 - "U" N-cad-Fc)Image 2698 (Fig 2 - Figure 2 - U373)Image 3353 (Fig 4 - Figure 4 - CytoD)Image 2158 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3089 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2705 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Fig 2 - Figure 2 - U373Image 3173 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2696 (Fig 2 - Figure 2 - U373)Image 2245 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2255 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3266 (Fig 3 - "U" N-cad-Fc)Image 2118 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3635 (Fig 4 - Figure 4 – Enucleation)Image 3087 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3299 (Fig 4 - Figure 4 - CytoD)Image 2510 (Fig 2 - Figure 2 - si control)Image 2966 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2015 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3731 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2995 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2910 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3615 (Fig 4 - Figure 4 – Enucleation)Image 1893 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2582 (Fig 2 - Figure 2 - si N-cad1)Image 1915 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2751 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2054 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3537 (Fig 4 - Figure 4 – CytD + Noco)Image 2650 (Fig 2 - Figure 2 - U373)Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).Image 3443 (Fig 4 - Figure 4 - CytoD)Image 2875 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2880 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3034 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2091 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2505 (Fig 2 - Figure 2 - si control)Image 3322 (Fig 4 - Figure 4 - CytoD)Image 3249 (Fig 3 - "U" N-cad-Fc)Image 2847 (Fig 2 - si N-cad1 (migration))Image 2496 (Fig 2 - Figure 2 - si control)Image 1934 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 1948 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3260 (Fig 3 - "U" N-cad-Fc)Image 3455 (Fig 4 - Figure 4 - CytoD)Image 2852 (Fig 2 - si N-cad1 (migration))Image 2256 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2720 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2068 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 3057 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2928 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3515 (Fig 4 - Figure 4 - Noco)Image 3559 (Fig 4 - Figure 4 – CytD + Noco)Image 2145 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2579 (Fig 2 - Figure 2 - si N-cad1)Image 3114 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3640 (Fig 4 - Figure 4 – Enucleation)Image 2629 (Fig 2 - Figure 2 - si N-cad1)Image 2104 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3334 (Fig 4 - Figure 4 - CytoD)Image 2132 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2097 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2905 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2658 (Fig 2 - Figure 2 - U373)Image 3530 (Fig 4 - Figure 4 - Noco)Image 3637 (Fig 4 - Figure 4 – Enucleation)Image 2330 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 1965 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2577 (Fig 2 - Figure 2 - si N-cad1)Image 2627 (Fig 2 - Figure 2 - si N-cad1)Image 2032 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2673 (Fig 2 - Figure 2 - U373)Image 2270 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3263 (Fig 3 - "U" N-cad-Fc)Image 2001 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2316 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3590 (Fig 4 - Figure 4 – CytD + Noco)Image 3727 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2533 (Fig 2 - Figure 2 - si control)Image 3587 (Fig 4 - Figure 4 – CytD + Noco)Image 3700 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2744 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2933 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2664 (Fig 2 - Figure 2 - U373)Image 2898 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2172 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2257 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2584 (Fig 2 - Figure 2 - si N-cad1)Image 2634 (Fig 2 - Figure 2 - si N-cad1)Image 3482 (Fig 4 - Figure 4 - Noco)Image 2528 (Fig 2 - Figure 2 - si control)Image 1977 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2862 (Fig 2 - si N-cad1 (migration))Image 2044 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3565 (Fig 4 - Figure 4 – CytD + Noco)Image 3256 (Fig 3 - "U" N-cad-Fc)Image 3239 (Fig 3 - "U" N-cad-Fc)Image 3476 (Fig 4 - Figure 4 - Noco)Image 2632 (Fig 2 - Figure 2 - si N-cad1)Image 2580 (Fig 2 - Figure 2 - si N-cad1)Image 2981 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2083 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2541 (Fig 2 - Figure 2 - si control)Image 3134 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3019 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3001 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3501 (Fig 4 - Figure 4 - Noco)Image 3133 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2230 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3024 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2630 (Fig 2 - Figure 2 - si N-cad1)Image 3658 (Fig 4 - Figure 4 – Enucleation)Image 1994 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 1951 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2103 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2857 (Fig 2 - si N-cad1 (migration))Image 2303 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2644 (Fig 2 - Figure 2 - U373)Image 2809 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3723 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2500 (Fig 2 - Figure 2 - si control)Image 2920 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3614 (Fig 4 - Figure 4 – Enucleation)Image 3510 (Fig 4 - Figure 4 - Noco)Image 3750 (Fig 4 - Figure 4 – Enucleation + Noco)Image 3320 (Fig 4 - Figure 4 - CytoD)Image 3164 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 1950 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2575 (Fig 2 - Figure 2 - si N-cad1)Image 3085 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3496 (Fig 4 - Figure 4 - Noco)Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.Image 2144 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2915 (Fig 2 - si N-cad1 + GFP-N-cad)Image 3098 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3609 (Fig 4 - Figure 4 – Enucleation)Image 2284 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3636 (Fig 4 - Figure 4 – Enucleation)Image 3006 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3445 (Fig 4 - Figure 4 - CytoD)Image 3090 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2667 (Fig 2 - Figure 2 - U373)Image 2768 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2757 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 2058 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2775 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 3154 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3324 (Fig 4 - Figure 4 - CytoD)Image 3641 (Fig 4 - Figure 4 – Enucleation)Image 1993 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2625 (Fig 2 - Figure 2 - si N-cad1)Image 2888 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2157 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2117 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2269 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2302 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3250 (Fig 3 - "U" N-cad-Fc)Image 3524 (Fig 4 - Figure 4 - Noco)Image 1980 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2052 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2231 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 1910 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2943 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2099 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2229 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3062 (Fig 2 - si N-cad1 + E-cad-GFP)Image 3365 (Fig 4 - Figure 4 - CytoD)Image 2523 (Fig 2 - Figure 2 - si control)Image 3586 (Fig 4 - Figure 4 – CytD + Noco)Image 2718 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3246 (Fig 3 - "U" N-cad-Fc)Image 2271 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 3113 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3184 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3518 (Fig 4 - Figure 4 - Noco)Image 1964 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 2693 (Fig 2 - Figure 2 - U373)Image 3029 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2732 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3363 (Fig 4 - Figure 4 - CytoD)Image 3253 (Fig 3 - "U" N-cad-Fc)Image 3659 (Fig 4 - Figure 4 – Enucleation)Image 2473 (Fig 2 - Figure 2 - si control)Image 2056 (Fig 1 - Figure 1C and 1D - Grouped Cells with No Free Edge.)Image 2803 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2315 (Fig 1 - Figure 1C and 1D - Isolated Cells (circle).)Image 2700 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3011 (Fig 2 - si N-cad1 + E-cad-GFP)Image 2029 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3682 (Fig 4 - Figure 4 – Enucleation + Noco)Fig 2 - si N-cad1 + E-cad-GFPImage 3564 (Fig 4 - Figure 4 – CytD + Noco)Image 3725 (Fig 4 - Figure 4 – Enucleation + Noco)Image 1904 (Fig 1 - Figure 1C and 1D - Migrating Cells. Pan-cadherin (green), pericentrin (red), Hoechst (blue).)Image 2171 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2687 (Fig 2 - Figure 2 - U373)Image 2810 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2938 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2202 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 3103 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3174 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 2971 (Fig 2 - si N-cad1 + GFP-N-cad)Image 2031 (Fig 1 - Figure 1C and 1D - Grouped Cells with Free Edge.)Image 3702 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2725 (Fig 2 - Figure 2 - Astroctyes (-) Calcium)Image 3088 (Fig 3 - Figure 3 - Circle N-cad-Fc)Image 3748 (Fig 4 - Figure 4 – Enucleation + Noco)Image 2782 (Fig 2 - Figure 2 - Astrocytes (+) Calcium)Image 2131 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2184 (Fig 1 - Figure 1C and 1D - Isolated Cells (quadrant).)Image 2518 (Fig 2 - Figure 2 - si control)Image 3441 (Fig 4 - Figure 4 - CytoD)Emeline CamandIsabelle DupinSandrine Etienne-MannevillefalseClassical cadherins control nucleus and centrosome position and cell polarity2009-06-01T11:17:05Z2018-11-29T11:17:05Z2018-11-29T11:17:05ZS-JCBD-20081203410.1083/jcb.200812034