bioimagesUnknownRobin W. KlemmThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200901145JCB

sec6-4 cells expressing the immunoisolation bait Gap1GFPLTLM9 and the soluble secreted marker InvRFP from the inducible promoter Gals. Gap1GFPLTLM9 localizes to dot-like structures which is typical for the Golgi apparatus and endosomes in yeast. InvRFP is accumulating intracellularly at the restrictive temperature 37˚C. It is not secreted (shown also biochemically in Fig S3B).

sec6-4 cells expressing the immunoisolation bait FusMidGFPLTLM9 and the soluble secreted marker InvRFP from the inducible promoter Gals. FusMidGFPLTLM9 localizes to the plasma membrane at permissive temperature 24˚C. InvRFP is not accumulating intracellularly at the permissive temperature 24˚C. Instead it is secreted (also shown biochemically in Fig S3B).

sec6-4 cells expressing the immunoisolation bait FusMidGFPLTLM9 and the soluble secreted marker InvRFP from the inducible promoter Gals. The immunoisolation bait FusMidGFPLTLM9 is accumulating intracellularly at the restrictive temperature 37˚C. <br />InvRFP is accumulating intracellularly at the restrictive temperature 37˚C. It is not secreted (shown also biochemically in Fig S3B).

sec6-4 cells expressing the immunoisolation bait Gap1GFPLTLM9 and the soluble secreted marker InvRFP from the inducible promoter Gals. Gap1GFPLTLM9 localizes to dot-like structures which is typical for the Golgi apparatus and endosomes in yeast. InvRFP is not accumulating intracellularly at the permissive temperature 24˚C. Instead, it is secreted (shown also biochemically in Fig. S3B).

bioimagesImage 1583 (Figure 1 - B)Image 1582 (Figure 1 - B)Image 1581 (Figure 1 - A)Figure 1 - AImage 1580 (Figure 1 - A)Figure 1Figure 1 - BMathias J. GerlCharles FergusonAndrej ShevchenkoChrister S. EjsingTomasz J. ProszynskiQuentin de RobillardMichal A. SurmaKai SimonsRobin W. KlemmJulio L. SampaioHermann-Josef KaiserfalseSegregation of sphingolipids and sterols during formation of secretory vesicles at the trans-Golgi network2009-05-11T11:17:29Z2018-11-29T11:17:29Z2018-11-29T11:17:29ZS-JCBD-20090114510.1083/jcb.200901145