{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Zhuojin Xu"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200902146"],"attach_to":["JCB"],"legend":["Imaging analyses of perinuclear mDpy-30 in HeLa cells. Cells were fixed (3%<br />formaldehyde), permeabilized (0.1% saponin), immuno-stained, and visualized using<br />confocal microscopy.<br /><br />Colocalization analysis of mDpy-30 (red) and TGN46 (green). In the published paper, a representative image from the Z stack is shown, here we include the full z-stack.<br /><br />Pearson’s correlations were<br />calculated from each image in the z-stack of confocal microscopy images of the perinuclear<br />regions (defined by p115 or TGN46) using the colocalization analysis from Image-Pro Plus 3.6<br />software (Student’s T-test, p = 5.02393E-12, n = 14). “Nu”: nucleus.","Imaging analyses of perinuclear mDpy-30 in HeLa cells. Cells were fixed (3%<br />formaldehyde), permeabilized (0.1% saponin), immuno-stained, and visualized using<br />confocal microscopy. <br /><br />Colocalization analysis of mDpy-30 (red) and p115 (green). In the published paper, a representative image from the Z stack is shown, here we include the full z-stack. <br /><br />Pearson’s correlations were<br />calculated from each image in the z-stack of confocal microscopy images of the perinuclear<br />regions (defined by p115 or TGN46) using the colocalization analysis from Image-Pro Plus 3.6<br />software (Student’s T-test, p = 5.02393E-12, n = 14). “Nu”: nucleus."],"repository":["bioimages"],"figure_sub":["Image 4100 (Figure 1 - C)","Figure 1","Image 4099 (Figure 1 - C)","Figure 1 - C"],"pubmed_authors":["Dezhi Mu","Zhuojin Xu","Bin Xia","Chris Mugler","Marc Zimmermann","Dzwokai Ma","Brian Matsumoto","Matthew Seaman","Benjamin Groves","Qiang Gong"],"additional_accession":[]},"is_claimable":false,"name":"A role of histone H3 lysine 4 methyltransferase components in endosomal trafficking","description":null,"dates":{"release":"2009-08-03T11:17:35Z","modification":"2018-11-29T11:17:35Z","creation":"2018-11-29T11:17:35Z"},"accession":"S-JCBD-200902146","cross_references":{"doi":["10.1083/jcb.200902146"]}}