bioimagesUnknownZhuojin XuThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200902146JCB

Imaging analyses of perinuclear mDpy-30 in HeLa cells. Cells were fixed (3%<br />formaldehyde), permeabilized (0.1% saponin), immuno-stained, and visualized using<br />confocal microscopy.<br /><br />Colocalization analysis of mDpy-30 (red) and TGN46 (green). In the published paper, a representative image from the Z stack is shown, here we include the full z-stack.<br /><br />Pearson’s correlations were<br />calculated from each image in the z-stack of confocal microscopy images of the perinuclear<br />regions (defined by p115 or TGN46) using the colocalization analysis from Image-Pro Plus 3.6<br />software (Student’s T-test, p = 5.02393E-12, n = 14). “Nu”: nucleus.

Imaging analyses of perinuclear mDpy-30 in HeLa cells. Cells were fixed (3%<br />formaldehyde), permeabilized (0.1% saponin), immuno-stained, and visualized using<br />confocal microscopy. <br /><br />Colocalization analysis of mDpy-30 (red) and p115 (green). In the published paper, a representative image from the Z stack is shown, here we include the full z-stack. <br /><br />Pearson’s correlations were<br />calculated from each image in the z-stack of confocal microscopy images of the perinuclear<br />regions (defined by p115 or TGN46) using the colocalization analysis from Image-Pro Plus 3.6<br />software (Student’s T-test, p = 5.02393E-12, n = 14). “Nu”: nucleus.

bioimagesImage 4100 (Figure 1 - C)Figure 1Image 4099 (Figure 1 - C)Figure 1 - CDezhi MuZhuojin XuBin XiaChris MuglerMarc ZimmermannDzwokai MaBrian MatsumotoMatthew SeamanBenjamin GrovesQiang GongfalseA role of histone H3 lysine 4 methyltransferase components in endosomal trafficking2009-08-03T11:17:35Z2018-11-29T11:17:35Z2018-11-29T11:17:35ZS-JCBD-20090214610.1083/jcb.200902146