{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Yoko Hayashi-Takanaka"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200904137"],"attach_to":["JCB"],"legend":["Fibroblasts were loaded with FabAuB-488 (w=0; green) and Fab311-555 (w=1; magenta). 5 z-stack images at 0.75 micro-m were captured every 3 min using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 100× (NA = 1.4) oil-immersion objective lens.","HeLa cells were loaded with Fab311-488. Images were collected every 0.4 s for 120 s using FV-1000 (Olympus) with a PlanSApo 60x (NA = 1.35). A 2 mincro-m spot was bleached between frames 10 and 11.","hTERT-RPE1 cells were loaded with GST-NLS-GFP (w=1; green) and Fab311-555 (w=2; red). Phase-contrast (w=0; gray) and fluorescence images were captured at 2 min intervals using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens. The hTERT-RPE1 cell exhibited chromosome missegregation after the transient exposure to ZM.","HeLa cells were stained with Fab311-488 (w=520; red) and anti-aurora B (w=567; green), and DAPI (w=461; blue). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35). Missegregated chromosomes were highlighted with Fab311-488.","Phase-contrast (w-0, gray) and Fab311-488 (w=1, green) images of three focal planes were captured at 3 min intervals for 36 h using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens. One of the cells went through 3 cell divisions, suggesting that Fab311-488 has little effect on cell division.","A hTER-RPE cell was stained  with Fab311-488 (w=520; cyan), anti-Rad21 (w=567; red), and DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35). Rad21 does not colocalize with the Fab311-488 foci.","Fibroblasts were incubated in BrdU for 10 min, fixed, and immunolabeled for aurora B (w=520; blue), H3S10ph (CMA311; w=567; red) and BrdU (w=664; blue). DNA was counterstained with DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35).","HeLa cells, which were loaded with Fab311-488 and imaged in live, were fixed and immunolabeled with Fab311-555. DNA was counterstained with DAPI. 9 z-stack images at 0.2 micro-m intervals were captured to select a plane comparable to the live image just before fixation. Phase-contrast (w=0; gray), Fab311-488 (w=1; green), Fab311-555 (w=2; red), and DAPI (w=3; blue) images were captured using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens.","HeLa cells were stained with Fab311-488 (w=520; red), anti-aurora B (w=567; green), and DAPI (w=461; blue). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35). Chromosome bridge was highlighted with Fab311-488.","HeLa cells were loaded with FabAuB-488 (w=0; green) and Fab311-555 (w=1; magenta). 5 z-stack images at 0.75 micro-m intervals were captured every 3 min using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 100× (NA = 1.4) oil-immersion objective lens. TMC was added after the 12th frame.","hTERT-RPE1 cells were loaded with GST-NLS-GFP (w=1; green) and Fab311-555 (w=2; red). Phase-contrast (w=0; gray) and fluorescence images were captured at 2 min intervals using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens. ZM was added after the 2nd frame and removed before 15th frame. Fab311-555 foci in the first 2 frames disappear soon after the addition of ZM, and Fab311-555 remains diffused even after the removal of ZM. Fab311-555 foci appear just before the nuclear membrane breaks down, which is indicated by GST-NLS-GFP diffusing out of the nucleus.","A hTER-RPE cell was stained  with Fab311-488 (w=520; cyan), anti-Sgo1 (w=567; red), and DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35). Sgo1 appears to be surrounded with Fab311-488.","HeLa cells were loaded with Fab311-488. Phase-contrast (w=0; gray) and Fab311-488 (w=1; green) images of three focal planes (2 micro-m intervals) were captured at 3 min intervals using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens. Fab311-488 initially diffuses throughout the nucleus and concentrates in foci, just before chromosomes condense. Fab311-488 remains concentrated on condensed chromosomes, and then diffuses again after chromosomes segregate.","Fibroblasts were loaded with Fab311-488. Phase-contrast (w=0; gray) and Fab311-488 (w=1; green) images were captured at 3 min intervals using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens. Fab311-488 is first concentrated in foci near the nuclear periphery, and several different foci appeared and disappeared repeatedly, long time before chromosomes began to condense.","Fibroblasts were loaded with FabAuB-488 (w=0; green) and Fab311-555 (w=1; magenta). 3 z-stack images at 0.75 micro-m  were captured every 2 min using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 100× (NA = 1.4) oil-immersion objective lens. ZM was added after the 4th frame.","A HeLa cell in anaphase was stained with Fab311-488 (w=520; green), Fab313-555 (w=567; red), anti-CENP-C (w=664; blue), and DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35).","A HeLa cell in metaphase was stained with Fab311-488 (w=520; green), Fab313-555 (w=567; red), anti-CENP-C (w=664; blue), and DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35).","HeLa cells expressing histone H2B-GFP (w=1; green) were loaded with Fab311-555 (w=2; red). Phase-contrast (w=0; gray)<br />and fluorescence images were taken every 3 min (3 images in the z-stack at 2-micro-m intervals). A missegregated chromosome  is easily visible in the Fab311-555 images, while contrast enhancement is required for detection in the H2B-GFP images.","HeLa cells were loaded with Fab311-488. Phase-contrast (w=0; gray) and Fab311-488 (w=1; green) images were captured at 3 min intervals using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens. Missegregated chromosomes are clearly detected and a micronucleus is formed after the cell division.","HeLa cells were stained with Fab311-488 (w=520; green) and  DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35).","A HeLa cell in G2 was stained  with Fab311-488 (w=520; green), Fab313-555 (w=567; red), anti-CENP-C (w=664; blue), and DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35).","HeLa cells were loaded with FabAuB-488 (w=0; green) and Fab311-555 (w=1; magenta). 3 z-stack images at 1.5 micro-m intervals were captured every 10 min using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 100× (NA = 1.4) oil-immersion objective lens.","A HeLa cell in prophase was stained with Fab311-488 (w=520; green), Fab313-555 (w=567; red), anti-CENP-C (w=664; blue), and DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35).","HeLa cells were loaded with Fab311-555 and Fab313-488. Phase-contrast (w=0; gray), Fab313-488 (w=1; red) and Fab311-555 (w=2; cyan) images were captured at 30 s intervals using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 100× (NA = 1.4) oil-immersion objective lens.","Fibroblasts were loaded with Fab311-488. Phase-contrast (w=0; gray) and Fab311-488 (w=1; green)  images were captured every 3 min using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 100× (NA = 1.4) oil-immersion objective lens. Tautomycetin (TMC) and ZM were added after the 5th and 16th frames, respectively.","HeLa cells were loaded with Fab311-488. Phase-contrast (w=0; gray) and Fab311-488 (w=1; green) images were captured at 3 min intervals using an inverted microscope (Ti-E; Nikon) with a PlanApo VC 60× (NA = 1.2) water-immersion objective lens. When Fab311-488 foci were detected in two frames, cells were fixed immediately.","HeLa cells were loaded with Fab311-488. Images were collected every 0.13 s for 12 s using FV-1000 (Olympus) with a PlanSApo 60x (NA = 1.35). A 2 mincro-m spot was bleached between frames 11 and 12.","HeLa cells were incubated in BrdU for 10 min, fixed, and immunolabeled for aurora B (w=520; blue), H3S10ph (CMA311; w=567; red) and BrdU (w=664; blue). DNA was counterstained with DAPI (w=461; gray). Images were acquired using a confocal microscope (FV1000; Olympus) with a 60x PlanSApo objective lens (NA = 1.35). H3S10ph signals are not seen in these cells."],"repository":["bioimages"],"figure_sub":["Image 5281 (Figure 4 - F)","Video 9","Video 3 - None","Video 6","Video 2 - None","Video 8","Video 6 - None","Video 7","Video 7 - None","Video 8 - None","Video 1 - None","Image 1628 (Figure 3 - B)","Image 5456 (Figure 3 - A)","Video 10 - None","Image 1626 (Figure 1 - E)","Video 2","Image 5425 (Figure 1 - F)","Image 5285 (Figure 4 - E)","Video 1","Video 3","Image 1607 (Video 6 - None)","Figure 1 - F","Image 5286 (Figure 4 - C)","Figure 1 - E","Image 1604 (Video 7 - None)","Image 1602 (Video 1 - None)","SupFigure 2 - None","Video 9 - None","Image 1603 (Video 2 - None)","Image 1624 (Figure 1 - E)","Image 1789 (SupFigure 1 - A)","Image 5287 (Figure 4 - C)","Image 1804 (SupFigure 1 - D)","SupFigure 1 - E","SupFigure 1 - D","SupFigure 1 - C","Figure 3 - A","SupFigure 1 - B","Figure 3 - B","SupFigure 1 - A","SupFigure 1","Image 1797 (SupFigure 2 - None)","SupFigure 2","Image 5458 (Video 8 - None)","Image 1805 (SupFigure 1 - C)","Figure 4 - E","Image 1608 (Figure 5 - B)","Figure 4 - F","Image 1606 (Video 3 - None)","Figure 4 - C","Image 1793 (SupFigure 1 - B)","Figure 4 - D","Image 1801 (SupFigure 1 - E)","Image 5284 (Figure 4 - D)","Figure 5 - B","Figure 5","Figure 4","Image 1798 (SupFigure 2 - None)","Image 5460 (Video 9 - None)","Figure 1","Image 1605 (Video 10 - None)","Image 5457 (Figure 1 - F)","Figure 3","Video 10","Image 1799 (Figure 3 - A)"],"pubmed_authors":["Yoko Hayashi-Takanaka","Kazuo Yamagata","Hiroshi Kimura","Naohito Nozaki"],"additional_accession":[]},"is_claimable":false,"name":"Visualizing histone modifications in living cells: spatiotemporal dynamics of H3 phosphorylation during interphase","description":null,"dates":{"release":"2009-12-07T11:17:53Z","modification":"2018-11-29T11:17:53Z","creation":"2018-11-29T11:17:53Z"},"accession":"S-JCBD-200904137","cross_references":{"doi":["10.1083/jcb.200904137"]}}