{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Preethi Vijayaraj"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200906094"],"attach_to":["JCB"],"legend":["The early epithelia ot the intestine is labelled with desmoplakin in the WT E9.5 embryo, vimentin is confined to the surrounding mesodermal tissue.","GLUT3 staining shows in yolk sac tissues in KO E9.5 embryos a severe mislocalization from the apical plasma membrane","Morphology and tissue integrity are completely intact in KO E9.5 embryos","The tight junctional marker occludin is localized apically in early intestine of WT embryos.","Double immunofluorescence of keratins and desmoplakin in the WT early intestine of E9.5 embryos. Fluorescence was performed on cryo material.","Vimentin stays restricted to the mesoderm of the yolk sac tissue in E9.5 keratin depleted embryos, it does not compensate for the missing keratin filament. DP is mislocalizes in the visceral endoderm.","Staining of WT yolk sac of  E9.5 embryos, demonstrating normal localization of keratins and desmosomes","Immunolabelling with GLUT1 of WT E9.5 yolk sac tissue shows a clear apical membrane  localization of the glucose transporters with an subapical keratin network directly underneath the apical plasma membrane","Immunolabelling of the tight junctional marker ZO-1 demonstates the normal apical dirstribution in the WT yolk sac","High magnification of yolk sac tissue in a E9.5 WT embryo.","No change in the apical distribution of ninein in keratin KO embryos compared to WT E 9.5, demonstrating that cell polarity is maintained.","The apical localization of the tight junctional marker occludin is maintained in the keratin KO embryos, supporting the observation that cell polarity is intact.","The glucose transporter GLUT3 is localized to the apical plasma membrane of yolk sac tissue in E9.5 WT embryos, keratins form a dense subapical filament.","Immunolablling of ZO-1 in the yolk sac tissue demonstrates that the tight junctions and therefore cell polarity is maintained in the epithelial KO tissues, as ZO-1 localizes as seen in WT tissues to the apical PM","Vimentin is restricted to the mesoderm in the E9.5 yolk sac tissue, desmoplakin clearly labels the epithelial visceral endoderm, however it is also expressed in the mesoderm of the yolk sac.","K19is expressed in the epithelia of the visceral endoderm in WT E9.5 yolk sac tissue.","H&E staining of a E9.5 embryo at high magnification","Ninein is associated with MTOC, and localizes apically in the early intestine of WT E9.5","Early intestine of a KO embryo shows complete tissue integrity","Section of a complete WT embryo at E9.5","Immunolabelling of the yolk sac tissue with the adherens junctional marker E-cadherin in cryosections.","GLUT1 is clearly mislocalized from the apical plasma membrane in the yolk sac tissue of keratin depleted E9.5 embryos","The high magnification of yolk sac tissue in an E9.5 keratin depleted embryo, shows no loss of tissue integrity of cell lysis.","Note keratin depletion in the yolk sac leads to a mislocalization of the desmosomes in the epithelial tissue of the visceral endoderm.","Staining with E-cadherin in KO yolk sac tissue shows no obvious alterations in the adherens junctions upon keratin depletion","In the early intestine in E9.5 embryos no keratins can be detected and DP shows a mislocalization.","K19 cannot be detected in the visceral endoderm of the keratin typeII cluster KO mice. K19 alone is unstable, since its binding partners K7 and K8 have been deleted. Therefore we have deleted all keratins in E9.5 embryos.","Corresponding to the WT early intestine in keratin depleted embryos, vimentin does not compensate in the epithelia tissue for the keratin filament, and is restricted to the surrounding mesodermal tissue, DP labels the intestinal tissue, however desmosomes are mislocalized in the KO embryos."],"repository":["bioimages"],"figure_sub":["Figure 2 - D'","Supp. Figure 2 - D","Supp. Figure 2 - C","Supp. Figure 2 - B","Image 8676 (Supp. Figure 2 - C)","Supp. Figure 2 - A","Image 8645 (Figure 5 - A'')","Image 8647 (Figure 5 - B'')","Image 8674 (Supp. Figure 2 - A)","Image 8646 (Figure 5 - B)","Figure 2 - E'","Image 8652 (Figure 3 - D)","Supp. Figure 1 - D","Figure 1 - I","Image 8655 (Figure 3 - E)","Image 8271 (Figure 2 - D)","Figure 5 - A''","Image 8678 (Figure 2 - B)","Figure 2 - E","Figure 2 - F","Figure 2 - F'","Image 8270 (Figure 2 - C')","Figure 2 - C","Figure 2 - D","Image 8442 (Figure 1 - I)","Figure 2 - A","Figure 2 - B","Image 8682 (Supp. Figure 1 - D)","Figure 3 - D","Figure 3 - E","Image 8597 (Figure 2 - F)","Figure 3 - F","Image 8268 (Figure 2 - A)","Image 8650 (Figure 3 - F)","Image 8548 (Figure 2 - E)","Image 8653 (Figure 3 - C)","Figure 3 - A","Figure 3 - B","Image 8675 (Supp. Figure 2 - B)","Figure 3 - C","Image 8269 (Figure 2 - C)","Image 8644 (Figure 5 - A)","Image 8636 (Figure 2 - F')","Image 8677 (Supp. Figure 2 - D)","Figure 5 - B''","Figure 5 - A","Image 8680 (Supp. Figure 1 - D)","Figure 2 - C'","Image 8504 (Figure 2 - D')","Figure 5 - B","Figure 5","Supp. Figure 2","Image 8567 (Figure 2 - E')","Image 8651 (Figure 3 - A)","Supp. Figure 1","Image 8463 (Figure 1 - I)","Figure 1","Image 8654 (Figure 3 - B)","Figure 3","Figure 2"],"pubmed_authors":["Rudolf E. Leube","Reinhard Windoffer","Preethi Vijayaraj","Cornelia Kröger","Thomas M. Magin","Ursula Reuter"],"additional_accession":[]},"is_claimable":false,"name":"Keratins regulate protein biosynthesis through localization of GLUT1 and -3 upstream of AMP kinase and Raptor","description":null,"dates":{"release":"2009-10-19T11:18:10Z","modification":"2018-11-29T11:18:10Z","creation":"2018-11-29T11:18:10Z"},"accession":"S-JCBD-200906094","cross_references":{"doi":["10.1083/jcb.200906094"]}}