bioimagesUnknownSerhiy PankivThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200907015JCB

Rab7Q67L can not recruit FYCO1(1156-1233) to the cytosolic vesicles.

LIR is essential for colocalization of FYCO1 with LC3B

The majority of FYCO1-decorated vesicles containing the selective autophagy substrate p62 are acidic (red only) as revealed by the use of a mCherry-YFP double tag fused to p62.

Overexpressed FYCO1 is enriched in the cell periphery, close to the microtubules plus end.

FYCO1 colocalizes with LC3B on the rim, but not inside of the vesical structures. HeLa cells co-transfected with GFP-FYCO1, mCherry-LC3B and myc-p62 were imaged by confocal microscopy 48h after transfection.

Rab7Q67L recruits FYCO1 1,038–1,233 to cytosolic vesicles

FYCO1 colocalizes with ATG16L on vesicles in MEFs (murine embryo fibroblasts).

FYCO1 decorated vesicles move along the microtubule tracks. HeLa cells transfected with mCherry-FYCO1 and GFP-tubulin were live-imaged by confocal microscopy 48h after transfection.

GFP-FYCO1 colocalizes with mCherry-LC3B

GFP-FYCO1 partially colocalizes with mCherry-ATG5

A tandem fusion of two FYVE domains from FYCO1 (2xFYVEFYCO1) localizes to the nuclear envelope and ER in HeLa cells.

bioimagesFigure 3 - DFigure 3 - EFigure 8 - CFigure 7 - A, top panelImage 10668 (Figure 2 - C, lower panel)Figure 8 - AFigure 7 - A, middle panelImage 10702 (Figure 5 - E)Figure 3 - CImage 10704 (Figure 7 - A, middle panel)Figure 2 - C, lower panelImage 10698 (Figure 3 - C)Image 10802 (Figure 7 - A, top panel)Image 10662 (Figure 3 - E)Image 10682 (Figure 2 - C, upper panel)Image 10714 (Figure 8 - C)Figure 2 - C, upper panelFigure 5 - EFigure 5Image 10661 (Figure 3 - E)Figure 7Figure 8Image 10712 (Figure 8 - A)Image 10715 (Figure 8 - C)Image 10696 (Figure 3 - C)Image 10659 (Figure 3 - D)Figure 3Figure 2Andreas BrechAud ØvervatnTrond LamarkGeir BjørkøySerhiy PankivJack-Ansgar BruunEndalkachew A. AlemuTerje JohansenfalseFYCO1 is a Rab7 effector that binds to LC3 and PI3P to mediate microtubule plus end–directed vesicle transport2010-01-25T11:18:14Z2018-11-29T11:18:14Z2018-11-29T11:18:14ZS-JCBD-20090701510.1083/jcb.200907015