{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Cédric Delevoye"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200907122"],"attach_to":["JCB"],"legend":["After internalization of Tf-A488 (green), control siRNA-treated cells were fixed and double labeled for AP-1 (γ-adaptin, red) and Rab11 (blue).","Spinning disc confocal microscopy was used to capture concomitant movements of Transferrin (Tf)-positive endosomes (green) and melanosomes(pseudo-colored in red) in melanocytic MNT-1 cells. Tf-A488 (10 μg/ml) was endocytosed continuously during the acquisition. Fluorescently labeled endosomes are distributed throughout the cell and frequently observed in close contact with melanosomes. Acquisition parameters were 200 msec exposure for the A-488 channel and 30 msec for bright field.","After internalization of Tf-A488 (green), KIF13A siRNA-treated cells were fixed and double labeled for AP-1 (γ-adaptin, red) and Rab11 (blue).","The MNT-1 cells were labeled for Rab11 (green) and AP-1 (γ-adaptin subunit, red) and analyzed by immunofluorescence microscopy after deconvolution.","Immunofluorescence microscopy analysis of MNT-1 cells treated with control siRNA that had internalized Tf-A488 (green) for 45 min and labeled for AP-1 (γ-adaptin subunit, red) and the cis-medial Golgi marker, GM130 (blue).","Immunofluorescence microscopy analysis of MNT-1 cells treated with  µ1A/ γ-adaptin siRNAs that had internalized Tf-A488 for 45 min (green) and labeled for AP-1 (γ-adaptin subunit, red) and the cis-medial Golgi marker, GM130 (blue).","MNT-1 control cells were fixed in cold methanol and endogenous KIF13A (red) localization was assessed relative to Tf internalized for 45 min (green) and microtubule network (β-tubulin, blue).","Immunofluorescence microscopy analysis of control siRNA-treated MNT-1 cells that were double labeled for AP-1 (γ-adaptin, green) and TGN46 (red).","The MNT-1 cells that had internalized Tf-A488 (green) for 45 min were labeled for AP-1 (γ-adaptin subunit, red) and analyzed by immunofluorescence microscopy after deconvolution.","The MNT-1 cells were labeled for EEA1 (green) and AP-1 (γ-adaptin subunit, red) and analyzed by immunofluorescence microscopy after deconvolution.","Immunofluorescence microscopy analysis of KIF13A (d-f) siRNA-treated MNT-1 cells that were double labeled for AP-1 (γ-adaptin subunit, green) and TGN46 (red).","MNT-1 control cells were fixed in cold methanol and endogenous KIF13A localization (green) was assessed relative to AP-1 (γ-adaptin subunit, red) ."],"repository":["bioimages"],"figure_sub":["Image 5245 (Figure 2 - Ai-l)","Figure 4 - Ee-h","Figure 2 - Ae-h","Image 5247 (Figure 3 - Af-j)","Figure 3 - Af-j","Figure 4 - Ae-h","Figure 2 - Aa-d","Figure 3 - Aa-e","Figure 4 - Aa-d","Image 5249 (Figure 4 - Ae-h)","Image 5250 (Figure 4 - Ea-d)","Figure 4 - Ea-d","Figure 4 - Fd-f","Image 5252 (Figure 4 - Fa-c)","Image 5243 (Figure 2 - Aa-d)","Figure 2 - Ai-l","Supplemental Video 1","Supplemental Video 1 - None","Image 5244 (Figure 2 - Ae-h)","Figure 4","Image 5248 (Figure 4 - Aa-d)","Image 5251 (Figure 4 - Ee-h)","Image 5253 (Figure 4 - Fd-f)","Image 5254 (Supplemental Video 1 - None)","Image 5246 (Figure 3 - Aa-e)","Figure 4 - Fa-c","Figure 3","Figure 2"],"pubmed_authors":["Jean-Baptiste Sibarita","Stéphanie Uzan-Gafsou","Graça Raposo","Danièle Tenza","Willie J.C. Geerts","Arie J. Verkleij","Ilse Hurbain","Hiroshi Ohno","Cédric Delevoye","Jean Salamero","Michael S. Marks"],"additional_accession":[]},"is_claimable":false,"name":"AP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis","description":null,"dates":{"release":"2009-10-19T11:18:21Z","modification":"2018-11-29T11:18:21Z","creation":"2018-11-29T11:18:21Z"},"accession":"S-JCBD-200907122","cross_references":{"doi":["10.1083/jcb.200907122"]}}