bioimagesUnknownCédric DelevoyeThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200907122JCB

After internalization of Tf-A488 (green), control siRNA-treated cells were fixed and double labeled for AP-1 (γ-adaptin, red) and Rab11 (blue).

Spinning disc confocal microscopy was used to capture concomitant movements of Transferrin (Tf)-positive endosomes (green) and melanosomes(pseudo-colored in red) in melanocytic MNT-1 cells. Tf-A488 (10 μg/ml) was endocytosed continuously during the acquisition. Fluorescently labeled endosomes are distributed throughout the cell and frequently observed in close contact with melanosomes. Acquisition parameters were 200 msec exposure for the A-488 channel and 30 msec for bright field.

After internalization of Tf-A488 (green), KIF13A siRNA-treated cells were fixed and double labeled for AP-1 (γ-adaptin, red) and Rab11 (blue).

The MNT-1 cells were labeled for Rab11 (green) and AP-1 (γ-adaptin subunit, red) and analyzed by immunofluorescence microscopy after deconvolution.

Immunofluorescence microscopy analysis of MNT-1 cells treated with control siRNA that had internalized Tf-A488 (green) for 45 min and labeled for AP-1 (γ-adaptin subunit, red) and the cis-medial Golgi marker, GM130 (blue).

Immunofluorescence microscopy analysis of MNT-1 cells treated with µ1A/ γ-adaptin siRNAs that had internalized Tf-A488 for 45 min (green) and labeled for AP-1 (γ-adaptin subunit, red) and the cis-medial Golgi marker, GM130 (blue).

MNT-1 control cells were fixed in cold methanol and endogenous KIF13A (red) localization was assessed relative to Tf internalized for 45 min (green) and microtubule network (β-tubulin, blue).

Immunofluorescence microscopy analysis of control siRNA-treated MNT-1 cells that were double labeled for AP-1 (γ-adaptin, green) and TGN46 (red).

The MNT-1 cells that had internalized Tf-A488 (green) for 45 min were labeled for AP-1 (γ-adaptin subunit, red) and analyzed by immunofluorescence microscopy after deconvolution.

The MNT-1 cells were labeled for EEA1 (green) and AP-1 (γ-adaptin subunit, red) and analyzed by immunofluorescence microscopy after deconvolution.

Immunofluorescence microscopy analysis of KIF13A (d-f) siRNA-treated MNT-1 cells that were double labeled for AP-1 (γ-adaptin subunit, green) and TGN46 (red).

MNT-1 control cells were fixed in cold methanol and endogenous KIF13A localization (green) was assessed relative to AP-1 (γ-adaptin subunit, red) .

bioimagesImage 5245 (Figure 2 - Ai-l)Figure 4 - Ee-hFigure 2 - Ae-hImage 5247 (Figure 3 - Af-j)Figure 3 - Af-jFigure 4 - Ae-hFigure 2 - Aa-dFigure 3 - Aa-eFigure 4 - Aa-dImage 5249 (Figure 4 - Ae-h)Image 5250 (Figure 4 - Ea-d)Figure 4 - Ea-dFigure 4 - Fd-fImage 5252 (Figure 4 - Fa-c)Image 5243 (Figure 2 - Aa-d)Figure 2 - Ai-lSupplemental Video 1Supplemental Video 1 - NoneImage 5244 (Figure 2 - Ae-h)Figure 4Image 5248 (Figure 4 - Aa-d)Image 5251 (Figure 4 - Ee-h)Image 5253 (Figure 4 - Fd-f)Image 5254 (Supplemental Video 1 - None)Image 5246 (Figure 3 - Aa-e)Figure 4 - Fa-cFigure 3Figure 2Jean-Baptiste SibaritaStéphanie Uzan-GafsouGraça RaposoDanièle TenzaWillie J.C. GeertsArie J. VerkleijIlse HurbainHiroshi OhnoCédric DelevoyeJean SalameroMichael S. MarksfalseAP-1 and KIF13A coordinate endosomal sorting and positioning during melanosome biogenesis2009-10-19T11:18:21Z2018-11-29T11:18:21Z2018-11-29T11:18:21ZS-JCBD-20090712210.1083/jcb.200907122