bioimagesUnknownRobert J. CainThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200907135JCB

C. TNF-alpha stimulated p110alpha or control siRNA transfected HUVECs<br />previously labelled with celltracker orange dye were grown on collagen matrices in<br />transwell chambers. Celltracker green-labelled THP-1 cells were added to HUVECs, and<br />TEM allowed to proceed toward an MCP-1 gradient in the lower chamber, for either 10<br />or 60 minutes before fixation. Fixed samples were stained with AlexaFluor 633-<br />conjugated phalloidin to visualize F-actin (grey) and z-stacks collected. Threedimensional<br />reconstructions were produced using Volocity software. A small gamma<br />correction was applied to the red channel in order to visualise weak cell tracker staining<br />at the edges of cell, which are difficult to distinguish upon 3D rendering

bioimagesFigure 5Image 5117 (Figure 5 - C)Image 5118 (Figure 5 - C)Figure 5 - CRobert J. CainBart VanhaesebroeckAnne J. RidleyfalseThe PI3K p110α isoform regulates endothelial adherens junctions via Pyk2 and Rac12010-03-22T11:18:23Z2018-11-29T11:18:23Z2018-11-29T11:18:23ZS-JCBD-20090713510.1083/jcb.200907135