{"database":"bioimages","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Shabeen Ally"],"journal":["The Journal of Cell Biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-JCBD-200908075"],"attach_to":["JCB"],"legend":["The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Kinesin heavy chain has been previously depleted by RNAi. GFP-tagged peroxisomes GFP-tagged peroxisomes do not move in microtubule-filled processes. Use Figure 1B (top panel) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.","The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Endogenous kinesin heavy chain has been previously depleted by RNAi. In addition, expression of peroxisome-targeted and truncated Unc104 has been induced with 5mM copper sulfate for a minimum of 12 hours. Expression of Unc104 restores peroxisome transport along the length of processes. Use Figure 2B (left) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.","The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. GFP-tagged peroxisomes can be seen moving along the length of microtubule-filled processes in a bi-directional manner. Use Figure 1B (top panel) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.","The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Dynein heavy chain has been previously depleted by RNAi. In contrast with control cells, GFP-tagged peroxisomes do not move in microtubule-filled processes. Use Figure 1B (top panel) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.","The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Endogenous kinesin heavy chain has been previously depleted by RNAi. In addition, expression of peroxisome-targeted and truncated kinesin-1 has been induced with 5mM copper sulfate for a minimum of 12 hours. Expression of truncated kinesin-1 restores peroxisome transport along the length of processes. Use Figure 3B (top left micrograph) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes."],"repository":["bioimages"],"figure_sub":["Image 9615 (Figure 2 - C)","Image 9613 (Figure 1 - B)","Image 9612 (Figure 1 - B)","Image 9614 (Figure 1 - B)","Figure 1","Figure 2 - C","Figure 3","Figure 3 - B","Image 9616 (Figure 3 - B)","Figure 1 - B","Figure 2"],"pubmed_authors":["Sarah E. Rice","Kari Barlan","Shabeen Ally","Vladimir I. Gelfand","Adam G. Larson"],"additional_accession":[]},"is_claimable":false,"name":"Opposite-polarity motors activate one another to trigger cargo transport in live cells","description":null,"dates":{"release":"2009-12-28T11:18:27Z","modification":"2018-11-29T11:18:27Z","creation":"2018-11-29T11:18:27Z"},"accession":"S-JCBD-200908075","cross_references":{"doi":["10.1083/jcb.200908075"]}}