bioimagesUnknownShabeen AllyThe Journal of Cell Biologyhttps://www.ebi.ac.uk/biostudies/studies/S-JCBD-200908075JCB

The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Kinesin heavy chain has been previously depleted by RNAi. GFP-tagged peroxisomes GFP-tagged peroxisomes do not move in microtubule-filled processes. Use Figure 1B (top panel) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.

The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Endogenous kinesin heavy chain has been previously depleted by RNAi. In addition, expression of peroxisome-targeted and truncated Unc104 has been induced with 5mM copper sulfate for a minimum of 12 hours. Expression of Unc104 restores peroxisome transport along the length of processes. Use Figure 2B (left) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.

The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. GFP-tagged peroxisomes can be seen moving along the length of microtubule-filled processes in a bi-directional manner. Use Figure 1B (top panel) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.

The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Dynein heavy chain has been previously depleted by RNAi. In contrast with control cells, GFP-tagged peroxisomes do not move in microtubule-filled processes. Use Figure 1B (top panel) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.

The time-lapse series is taken from a Drosophila S2 cell plated on a Concanavalin A coated substrate in the presence of 1uM Cytochalasin D. Endogenous kinesin heavy chain has been previously depleted by RNAi. In addition, expression of peroxisome-targeted and truncated kinesin-1 has been induced with 5mM copper sulfate for a minimum of 12 hours. Expression of truncated kinesin-1 restores peroxisome transport along the length of processes. Use Figure 3B (top left micrograph) as a marker for location of processes and cell body. Images were acquired every 1s for a period of 2 minutes.

bioimagesImage 9615 (Figure 2 - C)Image 9613 (Figure 1 - B)Image 9612 (Figure 1 - B)Image 9614 (Figure 1 - B)Figure 1Figure 2 - CFigure 3Figure 3 - BImage 9616 (Figure 3 - B)Figure 1 - BFigure 2Sarah E. RiceKari BarlanShabeen AllyVladimir I. GelfandAdam G. LarsonfalseOpposite-polarity motors activate one another to trigger cargo transport in live cells2009-12-28T11:18:27Z2018-11-29T11:18:27Z2018-11-29T11:18:27ZS-JCBD-20090807510.1083/jcb.200908075